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时间:2020-04-18
《大豆脂肪酸主要组分含量QTL定位-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、作物学报ACTAAGRONOMICASINICA2014,4O(9):1595—1603http://zwxb.chinacrops.org/ISSN0496-3490;CODENTSHPA9E—mail:xbzw@chinajourna1.net.caDOI:1O.3724/SP.J.1006.2014.01595大豆脂肪酸主要组分含量QTL定位邹筱韩粉霞陈明阳孙君明南金平闰淑荣杨华中国农业科学院作物科学研究所/农业部北京大豆生物学重点实验室/农作物基因资源与基因改良国家重大科学工程,北京10008l摘要:以中黄13x中黄20的10
2、0个BC2F2家系为作图群体,构建了一张包含131个SSR分子标记的遗传连锁图谱,图谱总长为2157.3cM,平均遗传距离为16.5cM,涵盖大豆的20个连锁群。利用气相色谱技术测定BC2F2、BC2F3和BC2F4回交群体的脂肪酸主要组分含量,采用IciMapping3.3完备区间作图法定位QTL。共检测到5种脂肪酸组分相关的QTL26个,与棕榈酸、硬脂酸、油酸、亚油酸和亚麻酸相关的QTL分别为5、5、7、5和4个;3个区间在不同年份被检测到与同一脂肪酸组分相关,sat294一vsatt228连续3年被检测到与棕榈酸含量相关,sat
3、253~satt323和sat292~satt397连续2年被检测到与油酸含量相关;4个区间被检测到与2种脂肪酸组分相关,其中sat294~satt228与棕榈酸、油酸相关,satt308~sat422与硬脂酸、亚油酸相关,sat292~satt397与油酸、亚油酸相关,satt374~satt269与油酸、亚麻酸相关。关键词:大豆;脂肪酸;遗传图谱;QTLQuantitativeTraitLociAssociatedwithMajorFattyAcidComponentsinSoy—beanZOUXiao,HANFen—Xia,CH
4、ENMing—Yang,SUNJun—Ming,NANJin—Ping,YANShu—Rong,andYANGHuaInstituteofCropScience,ChineseAcademyofAgriculturalSciences/KeyLaboratoryofSoybeanBiology(日e/ring),MinistryofAgriculture/Na—tionalKeyFacilityforCropGeneResourcesandGeneticImprovement,Beijing100081,ChinaAbstract:T
5、hesoybeanmappingpopulation(100BC2F2lines)wasdevelopedfromthecrossZhonghuang13xZhonghuang20.Thelinkagemapcontains131SSRmarkerscoveringatotaldistanceof2157.3cMwithanaverageof16.5cM.Fivefattyacidcomponentsweremeasuredbygaschromatographymethodinthreeyears,andQTLsassociatedw
6、iththesefattyacidcomponentswereanalyzedusingICIMmodelinsoftwareIciMapping3.3.Atotalof26OTLsweredetectedtobeassociatedwiththefivefattyacidcomponents,includingfiveQTLsforpalmiticacid,fiveQTLsforstearicacid,sevenQTLsforoleicacid,fiveQTLsforli—noleicacidandfourQTLsforlinole
7、nicacid.Threemarkerintervalsassociatedwiththesamefattyacidweredetectedrepeatedlyacrossyears,includingsat294-satt228forpalmiticaciddetectedinthreeyearsandsat253一satt323andsat292一satt397foroleicaciddetectedintwoyears.Fourintervalswerefoundtobeassociatedwithtwofattyacidcom
8、ponents。ie..sat294--satt228.rorpalmiticandoleicacids,satt308-sat422forstearicandlinoeicacids.sat292-satt397for
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