大豆粒形性状QTL的精细定位-论文.pdf

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1、作物学报ACTAAGRONOMICASINICA2013，39(4)：609—616http：／／www．chinacrops．org／zwxb／ISSN0496—3490；CODENTSHPA9E-mail：xbzw@chinajourna1．net．cnD0I：10．3724／SP．J．1006．2013．00609大豆粒形性状QTL的精细定位牛远谢芳腾布素红谢尚潜韩世凤耿青春刘兵章元明南京农业大学作物遗传与种质创新国家重点实验室，江苏南京210095摘要：在溧水中子黄豆×南农493—1衍生的504个F2：6家系中选择Satt331-Satt592目标区

2、间7个杂合单株和168个重组单株，衍生成356株RHL．F2个体(群体I)和168个重组体家系(群体II)。群体II来自142个F2：6家系，若每个F2：6家系只保留1个重组家系则构成群体III。采用lasso和复合区间作图(CIM)法检测3个群体粒形性状2种指标的QTL。结果表明，lasso法检测到的粒长关联标记是O19和s21／Satt331，而CIM检测到的QTL区间是S21-S22和023-019；lasso法检测到的粒宽关联标记是O19／021，而CIM检测到的QTL区间是023～O19／O19-O21；长宽比与s21～$22关联是由于粒长QTL引

3、起的，与023～O19／O19～021关联是由于粒长和粒宽QTL引起的。将原Satt331-Satt592目标区间的粒长QTL剖分为与标记$21～$22和023～O19／O19-021关联的2个多效性QTL。根据大豆基因注释数据库，Glymal0g35240和Glymal0g34980可能是控制粒形性状发育的候选基因。关键词：大豆：粒形：数量性状基因座；精细定位；候选基因FineMappingofQuantitativeTraitLociforSeedShapeTraitsinSoybeanNIUYuan，XIEFang—Teng，BUSu—Hong，XIE

4、Shang-Qian，HANShi—Feng，GENGQing—Chun，LIUBing，andZHANGYuan—MingStateKeyLaboratoryofCropGeneticsandGermplasmEnhancement，NanjingAgriculturalUniversity,Nanjing210095，ChinaAbstract：BasedonthetargetintervalregionofSSRmarkersSaR331-Satt592．weconstructed356RHL．F々individuals(popula．tionI)an

5、d168recombinantfamilies(populationII)derivedfrom504F26familiesofthedirectandreciprocalcrossesofLishuizhongzihuangwithNannong493—1tofinemapquantitativetraitsloci(QTLs)forseedlength(SL)，width(SW)，andlength-to—width(SLw)ratioinsoybeanusingcompositeintervalmapping(CIM)andlassoapproache

6、s．Amongthe168recombi—nantfamilies，onlyonerecombinantindividualwasselectedfromasameF，．familysothat142recombinantfamilieswerecon．structedaspopulationIII．ResponsevariableforQTLanalysiswasasorigina1observation．andthevaluecorrectedbytheassociatedmarkerinformation．Asaresult．markersassoci

7、atedwithSLwereO19andS21／Satt331fromlassomethodandS21-$22andO23—o19fromCIMmethod；markerassociatedwithSWwasO19／O21fromlassomethodandO23—019／Ol9一O21fromCIMmethod；andtheOTLassociatedwithmarkers$21-$22forSLWwasderivedfromQTLforSL，andtheQTLwithmarkers023—O19／O19—021forSLWwasderivedfromQT

8、LsforSLandSWThisindicatest