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时间:2020-04-18
《大豆油分含量稳定性QTL定位-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、分子植物育种,2014年,第l2卷,第2期,第254-261页MolecularPlantBreeding,2014,Vo1.12,No.2,254—261研究报告ResearchReport大豆油分含量稳定性QTL定位沈岩茹刘春燕’姜振峰王琳琳马占洲杨振,3辛大伟1蒋洪蔚胡国华zl4..陈庆山”1东北农业大学农学院,哈尔滨,150030;2黑龙江省农垦科研育种中心,哈尔滨,150090;3南京林业大学森林资源环境学院,南京,210037;4国家大豆工程技术研究中心,哈尔滨,150050·同等贡献作
2、者通讯作者,hugh757@vip.163.tom;qshchen@126.com摘要以美国半矮杆大豆品种Charleston为母本(早),中国品系东农594为父本(6)杂交,衍生的含147个重组自交系,2007~2009年分别在3个环境下种植,测定收获大豆种子油分含量。采用Shukla法计算油分含量的遗传稳定性,将稳定性结果利用复合区间模型和混合线性模型的复合区问作图法进行QTL分析。4个大豆油分稳定性QTL(sqOIL7-1,sqOIL8一,sqOIL15一J『和sqOIL12一J『)通过复合区
3、间作图法检测到,他们分别位于连锁群Dla、Dlb、J和G上,贡献率分别为5.33%、14.61%、5.83%和5.37%。混合区间作图法检测到4对上位性OTL,贡献率分别为5.65%、11.42%、15.19%和18.32%。研究结果对大豆油分含量重要基因的挖掘及油分相关性状的分子辅助育种提供了重要理论依据。关键词大豆,油分,QTL,稳定性QTLAnalysisofStabilityforOilContentinSoybeanShenYanru。‘LiuChunyan。‘JiangZhenfengW
4、angLinlinMaZhanzhouYangZhen1,3XinDaweiJiangHongweiHuGuohua2,4“ChenQingshan”1CollegeofAgriculture,NortheastAgriculturalUniversity,Harbin,150030;2TheCropResearchandBreedingCenterofLand—Reclamation,Harbin,150090;3CollegeofForestResourcesandEnvironment,Nan
5、jingForestryUniversity,Nanjing,210037;4TheNationalResearchCenterofSoybeanEngineeringandTechnology,Harbin,150050TheseauthorscontributedequallytothispaperCorrespondingauthors.hugh757@vip.163.tom;qshchen@126.comDOI:10.132718.mpb.012.000254AbstractTheF2:1F
6、2:14RILpopulationincluding147linesderivedfromacrossbetweenCharlestonan(AnAITIe—ricansemi-dwarfcultivar)andDongnong594(aChineseline)wereusedtoidentifiedquantitativetraitloci(QTL1associatedwithoilcontent.、)lplantedtheR/Lpopulationfrom2007to2009inthreedif
7、erentenvi-ronmentsTheseedswereharvestedforthemeasurementofoilcontentShuklaANOmethodwasusedtoanalyzethegeneticstabilityofoilcontent,andthwhichtherelatedQTLsweremappedbyCompositeIntervalMapping(CtM)andMixedCompositeIntervalMapping(MCIM).FourQTLs(sqOIL7-1
8、,sqOIL8-1,sqOIL15—1,andsqOIL12—1)onDla,Dlb,J,andGlinkagegroupsweredetectedforstabilityofoilcontentbyCIM,accountingfor5.33%,14.61%,5.83%,and5.37%ofthegeneralphenotypicvariation,respectively.ByMCIM,fourpairepistaitcQTLsweredetected,accoun
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