生物分离工程-双语版 2.ppt

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1、Chapter2Stepsinbioseparationprocesses1IntroductionBasicallythreestepsinbioseparationprocess(1)Celldisruption(2)Initialfractionation(3)High-resolutionfractionationEffectiveseparationprocessesforgeneticengineering:(1)Stable(2)Pure(3)Homogeneous(4)Activefo

2、rm(5)Highyield1IntroductionConsideringproteinpropertiesforbioseparation(1)size(2)charge(3)biologicalaffinity(4)hydrophobicity-solubilityProductsfrommammaliancelltechniques(1)enzyme(2)hormones(3)nonantibodyimmunoregulators(4)monoclonalantibodies(5)tumors

3、pecificantigens1IntroductionProteinfolding(1)shortrangedinteraction(secondarystructure)(2)gradualcombinationandreshuffling(重新组合）(subdomains,domains,andsubunitassemblies)Secondgenerationbiotechnologicalproducts(1)largermolecules(2)morecomplex(3)moreglyco

4、sylate(4)moresulphydrylbonds1IntroductionInitialbioseparationimportance(1)filtration(2)centrifugation(3)chromatographyClassifiedheuristics（论据，规则）(1)unitoperationmethods(2)designstepsequence(3)species(componentsproperties)(4)composition1IntroductionThree

5、factorsofdesigningbioseparationprocesses(1)purity(2)cost(3)marketMaterialstateandchoiceofseparationmethodsMaterialsecreted(ultrafiltration,centrifugation)Notsecretedmaterial(celldisruption,solid-liquidseparation)Materialinliquid(ultrafiltration,adsorpti

6、on)Materialinsolid(extractionintoaqueoussolution)NewandwordsProtactelawsuits诉讼Deleterious有害的，有毒的Vexing烦恼Hierarchic分等级Subdomain亚域Domains蛋白质结构的域Subunit亚单位Endoplasmicreticulum内质网Disulfidebond二硫键Academia学术界Delicate脆弱的Flowchart流程图Cytoplasmic细胞质的Periplasmic外周

7、胞质Osmotic渗透urea脲Guanidine胍IIProductexcretionfromthecellorcelldisruptionExcretedproteinadvantage(1)contaminationlevellow(2)easydownstreamprocessingsteps(3)notrequirecelldisruption(4)avoidhandlingviscousfluidsandproteinaserelease(5)facilityextractionofpro

8、ducts(6)signalpeptidedeterminessecretionCelldisruption(1)energy-intensive(强烈）andviolent(2)withoutdestroyinginterestprotein(3)contain(兼容）process(bacteria,yeast,mammalian,plant,andinsectsources)(4)becontrolled(5)bevalidatedProducts