基于抗体包被纳米金和铜配合物共固定修饰电极的癌胚抗原安培免疫传感器研究.pdf

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1、第23卷第5期传感技术学报Vo1．23No．52010年5月CHINESEJOURNALOFSENSORSANDACTUATORSMav2010OneAmperometricInllnunosensorsforCarcinoembryonicAntigeninHumanSerumBasedonAntibodyCoatedwithElectrolessPlatingNanoGoldandCopperComplexCoimmobilizedElectrodeWANGFeng，LIUFei，LITianhua，GANNing／1．NingboU

2、niversity，Ningb0Zh咖ng315211，China；＼2．Med&alExaminationSectionofNanfangHospitalofSouthernMedicalUniversity，Guangzhou510515，China)Abstract：Anovelamperometricimmunosensorforcarcinoembryonicantigen(CEA)inhumanserumwasestab—lishedbasedon[Cu(bpy)2(ONO)]NO3(CuL)andCEAantibodycoa

3、tedwithgoldnanoparticles(AuNPs)com—positefilm．Theglassycarbonelectrode(GCE)wasfirstlyoxidedandcarboxyelgroupwasformedonitssurface，andthenethylenediaminewasbondedtoit．TheammoniatedelectrodewassoakedinCuLandelectrolessgoldplat—ingsolution．ThenCuLcanbeabsorbedonGCEsurfaceby竹

4、一百stackinginteractionandAuNPsfilmof30—5OnmwereformedbyelectrolessgoldplatingtoformGCEfCuL—AuNPs．FurthermoreCEAantibody(antiCEA)wasabsorbedonthesurfaceofAuNPsfilmwithHRPemployedasblockingreagenttoformoneCEAimmunosensor(GCEICuL—AuNPs／antiCEA—HRP)．CuLcanbeemployedasanelectro

5、ntransfermediatorofcatalyticactivitydetectionofcarbamideperoxide(CP)，andHRPcouldenhancethesensitivityofimmunosensor．Aftertheimmu-nosensorwasincubatedwithCEAsolutionat37℃for30min．theelectrontransferaccessofCuLtoCPwaspartlyinhibited，whichleadtoalineardecreaseofthecatalytice

6、ficiencytoCPinpH6．5phosphatebuffersolution(PBS)，thedecreasepercentageofcurrenthadalinearrelationshipwiththeconcentrationofCEAbetween0．1and80ng／mL．Underoptimalconditions，thedetectionlimitforCEAis0．052ng／mL(3~r)．Thismethodsimplifiesthedetectionsystem．Ithasahighsensitivityan

7、dwidedetectionrangeforclinicalimmunoassayofCEAinpa—tients’serums．Keywords：[Cu(bpy)2(ONO)]NO3；electrolessplatingnanogold；CEA；reagentlessimmunosensorEEACC：7230J基于抗体包被纳米金和铜配合物共固定修饰电极的癌胚抗原安培免疫传感器研究术王峰，刘飞，李天华，干宁／1．宁波大学材料与化工学院，浙江宁波，315211；、＼2．南方医科大学南方医院检验科，广州，510515／摘要：研制了基于抗体包

8、被化学镀纳米金(AuNPs)和[Cu(bpy)(ONO)]NO，配合物(CuL)共固定修饰玻碳电极(GCE)的安培免疫传感器，并用于血清中癌胚抗原(CEA)的检测。首先将GCE电极表面氧化形成