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1、ArchivesofVirologyArchivesofVirology84,241--250{1985)©bySpringer-Verlag1985AnEntericCoronavirusoftheRabbit:DetectionbyImmunoeleetronMicroscopyandIdentifieationofStruetnralPolypeptides*ByJ.-P.DESC6TEAUX*,G.LUSSIER,L.BERTHIAUME,I~.ALAIN,C.SEGUIN,andM.TRUDELCentredeRechercheenV
2、irologic,InstitutArmand-Frappier,Laval,Quebec,CanadaWith3FiguresAcceptedAugust20,1984SummaryTheimmunoelectronmicroscopy(IEM)techniquehasbeenusedforthedetectionofarabbitentericcoronavirus(RECV).Immuneserumwaspreparedinguineapigs;theviralantigenusedfortheimmunizationprocedurew
3、asobtainedfromthecaecumofasickrabbit,concentratedbycentrifugationandpurifiedonPercollgradient.Inordertoidentifytheviralparticlesusedintheimmunizationprocedure,theproteinpatternoftheparticleswasdeterminedbyelectrophoresisandcomparedwiththepatternofotherknowncoronaviruses.Anal
4、ysisofstructuralpolypeptidesofthepurifiedviralparticlesrevealedapatternsimilartothatreportedforothercoronaviruses.Thesepolypeptidescrossreactedwithtwoothercoronavirusspecificimmunesera(IBVandTGE).IEMassayoffecalsamplescollectedfromhealthyandsickrabbitsshowedthepresenceofimmu
5、neaggregatesinspecimensfrombothsickandhealthyrabbits.Thoseaggregatescontainedviralparticulessharingmorphologicalcharacteristicswithothercoronaviruses.Furthermore,IEMassaywasshowntobemoresensitivethanadirectEMproceduretodetectcoronavirusparticlesinrabbitfeces.Thisassayalsoall
6、owedthedetectionofalargernumberofchroniccarriers.*ThisstudywassupportedbygrantsfromtheNSERC(no.G0965),Ottawa,Canada,andfromtheDepartmentd'AgricultureduQuebec(no.IAF-84-C-1114).242J.-1:).DESC6TEAUXetal.:IntroduetionTheelectronmicroscopy(E.M.)techniqueiscommonlyusedtodetectthe
7、presenceofcoronavirusparticlesinfecalsamplescollectedfromdifferentanimalspecies(3,8,ll,14,18)includingman(13).However,insuspensionsoffecalmaterials,numerousparticlesdonotalwaysexhibitthetypicalcoronavirusmorphologyandconsequentlyareoftenreferedtoasvirus-likeorcoronavirus-lik
8、eparticles(5,7).Thisisparticularlythecasewhenrabbitfecalmaterialisevaluated
