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1、BulletinofExperimentalBiologyandMedicine,Vol.137,No.2,February,2004197VIRUSOLOGYStructuralOrganizationoftheGenomeofSARS-AssociatedCoronavirus(StrainSoD)IsolatedontheTerritoryoftheRussianFederationG.G.Onishchenko*,V.M.Govorun,V.I.Sergienko,T.A.Akopian,K.T.Momynaliev,V.A.Ve
2、reshchagin,V.N.Lazarev,N.T.Vasil’ev*,V.I.Markov*,V.A.Merkulov*,V.A.Maksimov*,V.A.Mel’nikov*,andYu.M.LopukhinTranslatedfromByulleten’Eksperimental’noiBiologiiiMeditsiny,Vol.137,No.2,pp.222-224,February,2004OriginalarticlesubmittedDecember10,2003CompletenucleotidecDNAsequen
3、ce(29715nucleotides)ofSARS-associatedcoronavirus(strainSoD)isolatedforthefirsttimeintheterritoryoftheRussianFederationwasdetermi-ned.PhylogeneticanalysisrevealedmaximumsimilaritybetweenstrainSoDgenomeandFrankfurt1straingenome.Threenucleotidesubstitutionsdeterminingtwoamin
4、oacidsub-stitutionsweredetected.KeyWords:SARScoronavirus;Russianstrain;genomestructureAnewcoronavirusSARS-CoVcausingatypicalpneu-quences,thisagentformedanewgroupintheCoro-monia(severeacuterespiratorysyndrome)wasiso-navirusfamily.NewfeaturesweredetectedinthelatedinMarch,20
5、03,atvariouslaboratoriesoftheorganizationofSARS-CoVgenome,whichcanbeworld.AccordingtoWHO,thisdiseasewasregisteredbiologicallysignificant:short“anchor”ofglycopro-inmorethan25countriesoftheworld,includingRus-teinS,numberandlocationofminorORF,thepre-sia.Genomestructureofmore
6、than40strainsofsenceofsinglePLPprocopy.SARS-CoVisolatedfrompatientsindifferentcountri-Theaimofthisstudywasgeneticcharacterizationeswasidentified.Virusgenomeisaplus-senseRNAofCoDofSARSvirusisolatedattheterritoryofRus-consistingof30,000nucleotides,containing14opensiafromapa
7、tientwithatypicalpneumonia.readingframes(ORF)[2],5'capstructureand3'poly(A)tail.Thegenomeorganizationistypicalofcorona-MATERIALSANDMETHODSviruses:genesencodingreplicaseandfourstructuralproteinsarearrangedinasequence5'-rep-s-env-m-n-SoDstrainculturewasisolatedfromthethroat
8、and3'withshort3'and5'untranslatedregions.SARSnoseofapatientwithsuspectedatypicalpneumonia.agentg
