DB37∕T 3673-2019 牡蛎疱疹病毒1型检测技术规范 巢式PCR法

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1、ICS11.220B41DB37山东省地方标准DB37/T3673—2019牡蛎疱疹病毒І型检测技术规范巢式PCR法Ostreidherpesvirus-1DetectionSpecification-NestedPCR2019-08-30发布2019-09-30实施山东省市场监督管理局发布DB37/T3673—2019目次前言............................................................................II1范围.................................................

2、..............................12规范性引用文件.....................................................................13术语和缩略语.......................................................................13.1术语和定义.....................................................................13.2缩略语.......................

3、..................................................14试剂及仪器.........................................................................14.1试剂...........................................................................24.2仪器...........................................................................25采

4、样...............................................................................35.1采样对象.......................................................................35.2采样数量、方法和保存运输.......................................................35.3样品的采集........................................................

5、.............36检测...............................................................................36.1DNA的提取......................................................................36.2巢式PCR操作步骤...............................................................36.3电泳及测序..................................

6、...................................47结果...............................................................................47.1结果判定.......................................................................47.2结果表述.......................................................................4附录A（规范性附录）试剂及配制方法.

7、.............................................5附录B（资料性附录）检测目的片段的DNA序列及电泳图..............................7IDB37/T3673—2019前言本标准按照GB/T1.1—2009给出的规则起草。请注意本文件的某些内容可能涉及专利，本文件的发布机构不承担识别这些专利的责任。本标准由山东省农业农村厅提出并监督实施。本标准由山东省渔业标准化技术委员会(鲁TC03)归