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1、Preliminaryanalysisofgenomeofnosemabombycis潘国庆周泽扬向仲怀Itisjustthebeginningofthebigjob…--ThekeylabofSericultureofAgriculturalMinistry,SouthwestAgriculturalUniversity,Chongqing,P.R.China,400716.9/16/20211microsporidiaFirstrecognizedin1857,tillnow,hasbeen147years.Thefirstgen
2、omewaspublished2001,whichisEncephalitozooncuniculi,with2.9Mb.Foundinfromprotiststoinverbratesandallclassofvertebrate.Morethan140generaandalmost1200speciesrecognized.9/16/20212CharacteristicofthephylumExistonlyassporesoutsidethehostcellInvasionofhostcellinvolveseversiono
3、fpolartube.NomitochondriaandtypicalGolgiapparatus,andtheirribosomearenotoftypicaleukaryotictypebutresembletheribosomeofprokaryoticorganisms.9/16/20213ThebigquestionforevolutionarybiologistsAnimportantproblemtoresolveinordertodeterminethephylogeneticpositionofmicrosporid
4、iaiswhethertheyareprimitiveamitochondrial,havingsplitfromothereukaryotesbeforetheoriginofthemitochondria,orsecondarilyamitochondrial,beingderivedfromancestorswithmitochondria.9/16/20214ButwearemorethanitMoreresearcherinvesttheirenergyintothemicrosporidia,especiallythege
5、nusEncephalitozoon,whichisrelatedtohumandiseases.Ourpurposeisdividedintotwoparts,oneisforbasicresearch,todiscovertherelationshipbetweentheNbandsilkworm,NbandEc;theotheristhatweareeagertofindsomekeygenesusefultopreventtheNbdisease.9/16/20215backgroundNosemabombycisisthef
6、irstimportantpathogeninsilkwormfeeding.Nosemabombycistestistime&energy-consuminginsilkwormfeeding.9/16/20216NosemabombycisLineage(full)cellularorganisms;Eukaryota;Fungi/Metazoagroup;Fungi;Microsporidia;Apansporoblastina;Nosematidae;Nosema9/16/202179/16/202189/16/20219Li
7、ttleinformationavaliable9/16/202110Proteins entries in NCBI9/16/202111NbpurificationwithPercoll9/16/202112N.b全 基 因 组shotgun测 序 文 库 构 建N.bcompletegenomeDNAhydroshearKitextraction1.5-2kb2-2.5kbPUC18vectorPCRReactionForwardsequenceReversesequence9/16/2021131.Bascalling(Phr
8、edandPhd2fasta)2.VectorMark(crossmatch)3.Assemble(Phrap)4.Finishing(consed)5.RepeatMark,ORFPrediction,GeneAnno
