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1、AnInVitroStudyontheEffectsofNisinontheAntibacterialActivitiesof18AntibioticsagainstEnterococcusfaecalis1,2.1.1,2111ZhongchunTong,YuejiaoZhang,JunqiLing*,JingleiMa,LijiaHuang,LuodanZhang1DepartmentofOperativeDentistryandEndodontics,GuanghuaSchoolofStomatology,SunYat-senUniversity,G
2、uangzhou,Guangdong,China,2GuangdongProvincialKeyLaboratoryofStomatology,SunYat-senUniversity,Guangzhou,Guangdong,ChinaAbstractEnterococcusfaecalisrankamongtheleadingcausesofnosocomialinfectionsworldwideandpossessesbothintrinsicandacquiredresistancetoavarietyofantibiotics.Developme
3、ntofnewantibioticsislimited,andpathogenscontinuallygeneratenewantibioticresistance.Manyresearchersaimtoidentifystrategiestoeffectivelykillthisdrug-resistantpathogen.Here,weevaluatedtheeffectoftheantimicrobialpeptidenisinontheantibacterialactivitiesof18antibioticsagainstE.faecalis.
4、TheMICandMBCresultsshowedthattheantibacterialactivitiesof18antibioticsagainstE.faecalisOG1RF,ATCC29212,andstrainEweresignificantlyimprovedinthepresenceof200U/mlnisin.Statisticallysignificantdifferenceswereobservedbetweentheresultswithandwithout200U/mlnisinatthesameconcentrationsof
5、penicillinorchloramphenicol(p,0.05).ThecheckerboardassayshowedthatthecombinationofnisinandpenicillinorchloramphenicolhadasynergeticeffectagainstthethreetestedE.faecalisstrains.ThetransmissionelectronmicroscopeimagesshowedthatE.faecaliswasnotobviouslydestroyedbypenicillinorchloramp
6、henicolalonebutwasseverelydisruptedbyeitherantibioticincombinationwithnisin.Furthermore,assessingbiofilmsbyaconfocallaserscanningmicroscopeshowedthatpenicillin,ciprofloxacin,andchloramphenicolallshowedstrongerantibiofilmactionsincombinationwithnisinthanwhentheseantibioticswereadmi
7、nisteredalone.Therefore,nisincansignificantlyimprovetheantibacterialandantibiofilmactivitiesofmanyantibiotics,andcertainantibioticsincombinationwithnisinhaveconsiderablepotentialforuseasinhibitorsofthisdrug-resistantpathogen.Citation:TongZ,ZhangY,LingJ,MaJ,HuangL,etal.(2014)AnInVi
8、troStudyontheEffectsofNisinontheA
