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ID:40946640
大小:1013.67 KB
页数:253页
时间:2019-08-11
《MMB 150 Complement Methods and Protocols》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、MethodsinMolecularBiologyMethodsinMolecularBiologyTMVOLUME150ComplementComplementMethodsandMethodsandProtocolsProtocolsEditedbyEditedbyB.PaulMorganB.PaulMorganClassicalPathwayTerminalPathwayAlternativePathwayHUMANAPRESSHUMANAPRESSTheComplementSystem11TheComplementSystem:AnOverviewB.PaulMorgan1.Intro
2、ductionThecomplement(C)systemconsistsofagroupof12solubleplasmapro-teinsthatinteractwithoneanotherintwodistinctenzymaticactivationcas-cades(theclassicalandalternativepathways)andinthenonenzymaticassemblyofacytolyticcomplex(themembraneattackpathway)(Fig.1;Table1).Athirdactivationpathway,termedthelecti
3、npathway,hasrecentlybeendescribed(1,2).Controloftheseenzymaticcascades,essentialtopreventrapidconsumptionofCinvivo,isprovidedby10ormoreplasmaandmem-brane-boundinhibitoryproteinsactingatmultiplestagesofthesystem.Cplaysacentralroleininnateimmunedefense,whichprovidesasystemfortherapiddestructionofawide
4、rangeofinvadingmicroorganisms.ThepurposeofthisvolumeistoprovideabalancedaccountofthemethodswhichhavebeenappliedtothestudyoftheCsysteminclinicalandresearchlaboratories.Intheearlychapters,methodsfortheisolationoftheindividualCcomponents,regulatorsandreceptorswillbedescribedandassaysforthemea-suremento
5、fCactivityinthevariouspathwayswillbedetailed.LaterchapterswilldescribeaspectsofCmethodologyofrelevancetotheclinicallabora-tory—protocolsforscreeningforCdeficiencyanddeficiencyofC1inhibitor,methodsformeasurementofCactivationproductsinbiologicalfluidsandtis-suesandmethodsforallotypingCcomponents.Other
6、chapterswilldescribeadvancedtechnologieswhicharenowhavingenormousinfluenceonCresearch—structuralanalysisofthecomponentsandregulatorsandgenetargetingtogen-erateanimalsdeficientinindividualcomponentsandregulators.Inordertoappreciatethemethodologiestobedescribed,itisessentialfirsttounderstandthebasicso
7、fthesystem.From:MethodsinMolecularBiology,vol.150:ComplementMethodsandProtocolsEditedby:B.P.Morgan©HumanaPressInc.,Totowa,NJ12MorganFig.1.Thecomplementsystemanditscontrol.TheconstituentpathwaysoftheCs
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