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1、MethodsinMolecularBiologyMethodsinMolecularBiologyTMVOLUME147AffinityChromatographyChromatographyMethodsandProtocolsMethodsandProtocolsEditedbyPascalBailon,GeorgeK.Ehrlich,WWen-JianFung,en-JianFung,andandWWolfgangBertholdolfgangBertholdHUMANAPRESSHUMANAPRESSAnOverviewofAffinityChro
2、matography11AnOverviewofAffinityChromatographyMeirWilchekandIrwinChaiken1.IntroductionAffinitychromatographyispervasivelyacceptedandusedasatoolinbio-medicalresearchandbiotechnology;yetitsoriginsonly30yearsagosome-timesseemdimmedinhistory.However,thepotentialofthistechnologycontinue
3、stostimulatecontinueddevelopmentandnewapplications.Havinganewbookonthismethodologyiseminentlyappropriatetoday.Andbeingabletointroducethisbookisourpleasure.Affinitychromatographyasitisknowntodaywasintroducedin1968byPedroCuatrecasas,ChrisAnfinsen,andMeirWilchek,oneoftheauthorsofthisc
4、hapter.Thoughfewrelatedmethodsweredescribedearlier,theconceptandimmensepowerofbiorecognitionasameansofpurificationwasintro-ducedfirstinthat1968paper(1)entitled“AffinityChromatography.”IfyouexaminetheMedlineDatabaseforhowmanytimes“affinitychroma-tography”hasappearedinthetitleofscien
5、tificpapers,youwillfindalmost30,000paperscited.Thismeansthat,overthepast30years,threepublishedpapersperdayhavefeaturedthistechnology.Moreover,300patentshavebeengrantedduringthelast2yearsalone.Inarecentreview(2),ChrisLowestatedthataffinitychromatographyisatechniqueusedin60%ofallpuri
6、ficationprotocols.Sowhatexactlyisaffinitychromatography—thetechniquetowhichthisbookisdevoted?2.AffinityChromatographyandItsApplicationsforPurificationAffinitychromatographyisbasedonmolecularrecognition.Itisarelativelysimpleprocedure.Anygivenbiomoleculethatonewishestopurifyusuallyha
7、saninherentrecognitionsitethroughwhichitcanrecognizeanaturalorartifi-cialmolecule.Ifoneoftheserecognitionpartnersisimmobilizedonapoly-From:MethodsinMolecularBiology,vol.147:AffinityChromatography:MethodsandProtocolsEditedby:P.Bailon,G.K.Ehrlich,W.-J.Fung,andW.Berthold©HumanaPressIn
8、c.,Totowa,NJ12WilchekandCh
