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1、JournalofPhysics:ConferenceSeriesPAPER•OPENACCESSRelatedcontent-Three-layeredpolyplexasamicroRNASequence-specificinhibitionofmicroRNA-130atargeteddeliverysystemforbreastcancergenetherapygenebyCRISPR/Cas9systeminbreastcancerYanLi,YuDai,XiaojinZhangetal.cellline-Low-levellasersonmicroRNAanduncou
2、plingprotein2mRNAlevelsinhumanbreastcancercellsKSCanuto,AFTeixeira,JARodriguesetTocitethisarticle:NurAininaAbdollahetal2017J.Phys.:Conf.Ser.851012037al.-DesigningsyntheticRNAfordeliverybynanoparticlesDominikaJedrzejczyk,EdytaGendaszewska-Darmach,RozaViewthearticleonlineforupdatesandenhancement
3、s.Pawlowskaetal.ThiscontentwasdownloadedfromIPaddress61.174.131.57on12/08/2018at06:30ISMP2016IOPPublishingIOPConf.Series:JournalofPhysics:Conf.Series1234567890851(2017)012037doi:10.1088/1742-6596/851/1/012037Sequence-specificinhibitionofmicroRNA-130agenebyCRISPR/Cas9systeminbreastcancercelllin
4、eNurAininaAbdollah¹,KumitaaThevaDas²,NarazahMohdYusoff³,SitiRazilaAbdulRazak¹¹OncologyandRadiologicalSciencesCluster,AdvancedMedicalandDentalInstitute,UniversitiSainsMalaysia,Bertam,13200KepalaBatas,PulauPinang,Malaysia.²InfectomicsCluster,AdvancedMedicalandDentalInstitute,UniversitiSainsMalay
5、sia,Bertam,13200KepalaBatas,PulauPinang,Malaysia.³RegenerativeMedicineCluster,AdvancedMedicalandDentalInstitute,UniversitiSainsMalaysia,Bertam,13200KepalaBatas,PulauPinang,Malaysia.E-mail:sitirazila@usm.myandaininaabdollah@gmail.comAbstract.MicroRNAs(miRNAs)areshortstrandednoncodingRNAthatplay
6、importantrolesinapoptosis,cellsurvival,developmentandcellproliferation.However,geneexpressioncontrolviasmallregulatoryRNA,particularlymiRNAinbreastcancerisstilllessexplored.Therefore,thisprojectaimstodevelopanapproachtotargetmicroRNA-130ausingtheClusteredRegularlyInterspacedShortPalindromicRep
7、eat(CRISPR)/Cas9systeminMCF7,breastcancercellline.The20bpsequencestargetatstemloop,3'and5'endofmiR130awereclonedintopSpCas9(BB)-2A-GFP(PX458)plasmid,andthepositivecloneswereconfirmedbysequencing.Atotalof5μgofPX458-miR130awastransfectedt
