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1、ChineseScienceBulletin©2007SCIENCEINCHINAPRESSARTICLESSpringerMechanismoftheinteractionbetweenAunano-particlesandpolymeraseinnanoparticlePCR1,31,321,2†1†MILiJuan,ZHUHongPing,ZHANGXiaoDong,HUJun&FANChunHai1ShanghaiInstituteofAppliedPhysics,ChineseAcademyofSciences,Shanghai20
2、1800,China;2Bio-XLifeResearchCenter,CollegeofLifeScienceandBiotechnology,ShanghaiJiaoTongUniversity,Shanghai200030,China;3GraduateUniversityofChineseAcademyofSciences,Beijing100049,ChinaNanoparticlePCRisanovelmethodtooptimizeDNAamplification.Itperformswellinimprovingspecifi
3、city,enhancingsensitivityandspeed.Severalmechanismswereproposedinpreviousstudies:onewasbasedontheinteractionbetweengoldnanoparticles(AuNPs)andDNAwhiletheotherwasattributedtotheheattransferpropertyofAuNPs.Inthispaper,weproposethattheinteractionbetweenAuNPsandDNApolymerasecan
4、significantlyinfluencePCR.First,theadditionofDNApolymerasecaneliminatetheinhibitoryeffectsofexcessAuNPs.Second,theadditionofAuNPswillincreaseyieldofthedesiredPCRproductandmaketheoptimumconcentrationofDNApolymerasemovetohighervalue.Third,whileexcesspolymerasemightinhibitampl
5、ificationefficiency,AuNPscanreversethisprocessandtheyieldofPCRamplification.BasedontheseresultsweproposeapossiblemechanismthatAuNPsmightmodulatetheactivityofpolymeraseandimprovePCRamplification.PCR,nanoparticles,Au/goldnanoparticle,polymerase,mechanism[6]Polymerasechainreac
6、tion(PCR),aninvitrotechniqueprotein)-assistedPCR.Later,Lietal.foundenhancedtoamplifyaregionofDNA,iswidelyusedingeneam-sensitivityandextensionrateofPCRwithAuNPs.[7]plification,analysisoforiginalDNAcopies,geneidenti-BasedonthermalconductivityofAuNPs,theypro-ficationandsubtypi
7、ng,anddiseasediagnosis[1―3].How-posedthatAuNPscouldshortenreactiontimeorin-creaseheating/coolingratesaccordingtoitsthermalef-ever,therearestillproblemsremainingtobesolved,e.g.ficiency,astheheatmighthelptomatchtheprimerandnonspecificamplificationresultingfrommismatchtemplate
8、.InmodulatingPCR,nanomaterialshavespe-betweentheprimerandtemplatehasbeenpuzzlingci
