实时定量pcr相对定量实验入门指南abi 7300-7500

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1、入门指南简介和相对定量相对定量实验示例实验美国应用生物系统公司7300/7500实时定量PCR仪设计相对定量实验PrimerExtendedonmRNA5′3′执行反转录5′cDNAReversePrimerOligod(T)orrandomhexamerSynthesisof1stcDNAstrand3′5′cDNA从相对定量反应板生成PCR数据在相对定量研究中生成PCR数据©Copyright2004,AppliedBiosystems.Allrightsreserved.ForResearchUseOnly.Notforus

2、eindiagnosticprocedures.AuthorizedThermalCyclerThisinstrument,SerialNo___________,isanAuthorizedThermalCycler.Itspurchasepriceincludestheup-frontfeecomponentofalicenseunderUnitedStatesPatentNos.4,683,195,4,683,202and4,965,188,ownedbyRocheMolecularSystems,Inc.,andunder

3、correspondingclaimsinpatentsoutsidetheUnitedStates,ownedbyF.Hoffmann-LaRocheLtd,coveringthePolymeraseChainReaction("PCR")processtopracticethePCRprocessforinternalresearchanddevelopmentusingthisinstrument.TherunningroyaltycomponentofthatlicensemaybepurchasedfromApplied

4、BiosystemsorobtainedbypurchasingAuthorizedReagents.ThisinstrumentisalsoanAuthorizedThermalCyclerforusewithapplicationslicensesavailablefromAppliedBiosystems.ItsusewithAuthorizedReagentsalsoprovidesalimitedPCRlicenseinaccordancewiththelabelrightsaccompanyingsuchreagent

5、s.PurchaseofthisproductdoesnotitselfconveytothepurchaseracompletelicenseorrighttoperformthePCRprocess.FurtherinformationonpurchasinglicensestopracticethePCRprocessmaybeobtainedbycontactingtheDirectorofLicensingatAppliedBiosystems,850LincolnCentreDrive,FosterCity,Calif

6、ornia94404.DISCLAIMEROFLICENSE:Norightsforanyapplication,includinganyinvitrodiagnosticapplication,areconveyedexpressly,byimplicationorbyestoppelunderanypatentorpatentapplicationsclaiminghomogeneousorreal-timedetectionmethods,includingpatentscoveringsuchmethodsusedinco

7、njunctionwiththePCRprocessorotheramplificationprocesses.The5'nucleasedetectionassayandcertainotherhomogeneousorreal-timeamplificationanddetectionmethodsarecoveredbyUnitedStatesPatentNos.5,210,015,5,487,972,5,804,375and5,994,056,ownedbyRocheMolecularSystems,Inc.;bycorr

8、espondingpatentsandpatentapplicationsoutsidetheUnitedStates,ownedbyF.Hoffmann-LaRocheLtd;andbyUnitedStatesPatentNos.5,538,84