资源描述：
《茶树花转录组微卫星分布特征》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、作物学报ACTAAGRONOMICASINICA2014,40(1):80−85http://zwxb.chinacrops.org/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2014.00080茶树花转录组微卫星分布特征*王丽鸳韦康张成才成浩中国农业科学院茶叶研究所/国家茶树改良中心,浙江杭州310008摘要:利用Perl语言,对茶树花转录组序列进行大通量SSR位点的发掘,发现含SSR的序列10290条,共12582个SSR,平均2.41kb出现一个SSR
2、。在茶树花的转录组中共发现340种碱基重复模式,所占比例最高的是(AG/CT)n(44.99%)。在49586条注释成功的茶树花Unigene中,共发现10490个SSR位点,其中位于编码区的1917个,其出现频率仅为0.102SSR/1000bp,而非编码区为3.072SSR/1000bp。在基因编码区中出现频率最高的是三碱基微卫星(1140,59.5%),其次是六碱基微卫星(524,27.3%)。茶树花转录组所含微卫星以重复长度小于20bp的序列最多,大于20bp的仅为25.2%。茶树花转录组中,含微卫星基因的平均表达水平显著低于不含微卫星基因,其中含复杂微卫星基因
3、的平均基因表达水平最低。关键词:茶树;微卫星;花;转录组CharacterizationofMicorsatellitesinTea(Camelliasinensis)FloralTranscriptome*WANGLi-Yuan,WEIKang,ZHANGCheng-Cai,andCHENGHaoTeaResearchInstitute,ChineseAcademyofAgriculturalSciences/NationalCenterforTeaImprovement,Hangzhou310008,ChinaAbstract:Themicrosatelliteso
4、rsimplesequencerepeats(SSRs)inCamelliasinensisfloraltranscriptomewerecharacterized.Atotalof12582SSRswereidentifiedin10290unigenes,withoneSSRper2.41kb.Amongall340SSRmotifs,(AG/CT)nwasthemostfrequentrepeatmotif(44.99%).Atotalof10409SSRsoccurredin49586unigeneswithBlastmatchestoannotatedprot
5、einsinfourdatabases,only1917ofwhichoccurredinprotein-codingregionsofthesesequences.ThedensityofSSRswasmuchhigherinnon-codingregionsthanincodingregions(0.102SSRsper1000basepairsincodingregionsvs.3.072innon-codingregions).Inthesixrepeatmotifs,tri-nucleotiderepeatswerethemostabundantincodin
6、gregions(1140),followedbyhexa-nucleotide(524)repeats.Themicrosatelliteswithlengthbelow20bpwereinmaximumproportion,whilethemicrosatellitesover20bpwereonly25.22%.Theexpressionlevelofgenescontainingmicrosatelliteswassignificantlylowerthanthatnotcon-tainingmicrosatellites.Theoverallexpressio
7、nlevelsofgenescontainingcompoundmicrosatelliteswerelowest.Keywords:Camelliasinensis,Microsatellites;Flower;Transcriptome微卫星(microsatellite)又称SSR(simplesequence致基因表达产物的改变,在选择作用下,编码区微repeat,简单重复序列),是真核生物基因组中的高度卫星发生频率比其他基因组区域低。如杨树基因组重复序列,是由少数几个核苷酸组成的串联重复序中编码区微卫星的发生频率仅为非编码区的1
