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ID:36567964
大小:39.00 KB
页数:11页
时间:2019-05-12
《创伤弧菌脓毒症大鼠肺组织脂多糖受体CD14mRNA、核因子κB的基因动态表达》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、创伤弧菌脓毒症大鼠肺组织脂多糖受体CD14mRNA、核因子-κB的基因动态表达作者:郑阿迈,陈玉熹,李萌芳,梁欢,卢中秋【摘要】目的:探讨创伤弧菌(Vibriovulnificus,VV)脓毒症大鼠肺组织脂多糖受体CD14mRNA、核因子-κB(NF-κB)基因的表达及其动态变化。方法:SD雄性大鼠60只,随机分为6组,每组10只。第1组:正常对照组(NC组);第2~6组为VV组:予大鼠下肢皮下注射VV,造成VV脓毒症模型,以注射VV后2、6、9、12、16h各时间点分别为2~6组。取各组大鼠部分肺组织,用RT-PCR法测定大鼠肺组织不同时间点CD14mRNA
2、、NF-κBmRNA的表达。结果:NC组大鼠肺组织有少量CD14、NF-κBmRNA表达,分别为0.392±0.056和0.366±0.179。感染VV后大鼠肺组织CD14mRNA表达增加,2、6、9、12、16h的表达量分别为0.976±0.180,1.148±0.218,1.026±0.033,0.988±0.129,0.970±0.125。大鼠肺组织NF-κBmRNA表达量也明显增加,2、6、9、12、16h的表达量分别为0.763±0.215,1.100±0.101,1.068±0.174,1.057±0.076和0.800±0.205。与NC组相比,
3、二者均明显升高(P<0.05)。结论:VV脓毒症大鼠肺组织CD14、NF-κB的基因表达水平在脓毒症中明显升高,其与脓毒症的发生、发展密切相关,动态监测其改变对VV脓毒症发病过程具有一定的预警意义。【关键词】创伤弧菌;脓毒症;脂多糖受体CD14;核因子-κ11B;基因Abstract:Objective:TodetecttheGeneexpressionandthedynamicchangeofpulmonarylipopolysaccharidereceptorCD14andNF-κBinratswithVibriovulnificus(VV)sepsis.
4、Methods:SixtymaleSprague-Dawley(SD)ratswereequallydividedintosixgroupsrandomly.Thefirstgroupwasnamedcontrolgroup.VVgroupsincludethesecondtosixthgroupwhichwereinjectedVVtomakeVVsepsismodel.Samplesweretakenfromcontrolgroupandthesecondgrouptothesixthgroupatthetimepointof2,6,9,12,16haft
5、erinjectionrespectively,thegenesofpulmonarylipopolysaccharidereceptorCD14andNF-κBinrats'lungweremeasuredinthedifferenttimebythemethodofRT-PCR.Results:TheGeneexpressionofpulmonarylipopolysaccharidereceptorCD14andNF-κBinthecontrolgroupwasalowlevel(0.392±0.056,0.366±0.179),buttheyincre
6、asedsignificantlyat2h(0.976±0.180,0.763±0.215),6h(1.148±0.218,1.100±0.101),9h(1.026±0.033,1.068±0.174),12h(0.988±0.129,1.057±0.076),16h(0.970±0.125,0.800±0.205)afterVVbeinginjected(P<0.05).Conclusion:OurdatasuggestthattheGeneexpressionofpulmonarylipopolysaccharidereceptorCD14andNF-κ
7、Belevationare11associatedwiththedevelopmentofVVsepsis.MonitoringthechangeoftheGeneexpressionofpulmonarylipopolysaccharidereceptorCD14andNF-κBinthelunghavethebenefitofmasteringtheVVsepsisprogress.Keywords:Vibriovulnificus;sepsis;CD14;NF-κB;gene创伤弧菌(Vibriovulnificus,VV)是一种高致死性嗜盐性G-弧菌,
8、可引起严重的伤口感染、脓毒症等[1]。
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