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1、类芽孢杆菌产絮凝多糖发酵条件优化及成分分析姜彬慧,李若男,李凤达,赵研,胡筱敏东北大学资源与土木工程学院，辽宁沈阳110004摘要：为提高微生物絮凝多糖的产量，应用响应面方法对一株高效产絮类芽孢杆菌（以下称A9）发酵产絮凝多糖的影响因素进行分析.结果表明：①根据P-B（Plackett-Burman）法确定，影响A9产絮凝多糖的显著因子为装瓶量、ρ(MgSO4·7H2O)和ρ(可溶性淀粉)；②通过Box-Behnken试验设计及响应面分析，确定了优化的培养基组成与培养条件，在ρ(可溶性淀粉)为17.18g/L，ρ(酵母膏)为3g/L，ρ(K2HPO4)为6g/L
2、，ρ(MgSO4·7H2O)为0.21g/L，ρ(NaCl)为0.1g/L，装瓶量为51.03mL/250mL，pH为8的优化条件下，絮凝多糖实际产量为2.49g/L，与理论预测值（2.50g/L）接近；③利用红外光谱法检测A9产絮凝多糖的特征基团分别为—OH、—COO-、—C—O—C—和NHCOCH3等极性基团；④结合A9对不同碳源利用的单因素试验结果，推测A9产絮凝多糖的主要成分分别为含有α-吡喃型糖苷键的甘露糖和葡萄糖、酸性多糖和乙酰氨基多糖等.关键词：絮凝多糖；响应面法；发酵条件优化；类芽孢杆菌中图分类号：Q89文献标识码A文章编号：1001-6929(
3、0000)00-00-00FermentationConditionsOptimizationandCompositionAnalysisofPolysaccharide-basedBioflocculantProducedbyPaenibacillussp.A9JIANGBin-hui,LIRuo-nan,LIFeng-da,ZHAOYan,HUXiao-minCollegeofResourcesandCivilEngineering,NortheasternUniversity,Shenyang110004,China.Abstract:Inordertoi
4、ncreasethemicrobialflocculatingpolysaccharideproductionbyPaenibacillussp.A9,anovelmicrobialstrainisolatedfromsoilsinShenyang,thePlackett-Burman(P-B)designandResponseSurfaceMethodology(RSM)wereappliedtooptimizetheinfluencefactorsinthefermentationcultureprocessbasedonsinglefactorexperi
5、ments.TheP-Bresultsshowedthatthesolublestarch,MgSO4·7H2OandamountofmediawerethethreemainfactorsthatsignificantlyaffectingthepolysaccharideproductionbyPaenibacillussp.A9inthelaboratory.AsecondordermodelofthepolysaccharideproductionbystrainA9wasobtainedbyBox-BehnkendesignandRSManalysis
6、.Resultsshowedthattheoptimalproductionofflocculatingpolysaccharideconditionwas17.18g/Lofsolublestarch,3g/Lofyeastextract,6g/LofK2HPO4,,0.21g/LofMgSO4·7H2O,0.1g/LofNaCl,51.03mL/250mLofmediaamountandpH8.0.Theresultsofverificationtestingshowedthattheoptimizedaverageproductionofflocculat
7、ingpolysaccharidewasis2.49g/L,veryclosedtothepredictedvalue2.50g/L,whichwashigherthanthatofthesinglefactorby18.07%.,TheinfraredspectrashowedthattheflocculatingpolysaccharideproducedbystrainA9mainlycontainspolargroups,suchashydroxyl(-OH),carboxyl(-COO-),methoxyl(-C-O-C-)andacetylamino
8、group(-NHCOC
