基于srap标记的国槐古树遗传多样性分析

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1、基于SRAP标记的国槐古树遗传多样性分析要反应株高与叶宽和叶轴间距有较大的负相关关系，即植株越高，叶片宽度和叶轴间距相对较大，该结论为国槐基于性状相关进行单株选择提供了有力基础。关键词：国槐；古树；SRAP；遗传多样性II山东农业大学全日制硕士专业学位论文StudyonGeneticDiversityofTheAncientSophoraJaponicaL．bySRAPAnalysisAbstractSophorajaponicaL．isatreespecieswithtimber,medicinal，edi

2、bleandornamentalvalues．extensiveintroductionandcultivationinotherpartsoftheworld．InordertobetterforappraisalofSophoraJaponicagermplasmresources，thispaperanalysedgeneticdiversityanddiscussedthegeneticsimilarityofSophoraJaponica、析mthemethodofSRAPmarkegItwasex

3、pectedthatresearchresultswouldprovidethebasisforthebreedingofnewvarietiesandgermplasmresources．Themainresultsfromthisstudyareasfollows：1、GenomicDNAWaSextractedfromleavesofbythemethodofSophorajaponicamodifiedCTAB．Weobtainedhi．曲qualityandpuritygenomicDNAwhich

4、cRnmeettherequestofPCRreaction．2、AnorthogonalofL16(45)designcombinedwithsinglefactorexperimentWasappliedtooptimizeSRAP-PCRamplificationsystemofSophorajaponica．TheeffectofthemainreactionsystemelementsonSRAP-PCRweretested．AmostsuitableSRAP—PCRsystemforSophora

5、japonica．W弧establishedthatistotal20I．tLreactionsystemcontainin：M92+2．0mmol／L，DNAtemplate80ng，dNTPs0．15mmol／L，primerllxrnoUL，10×PCRbuffer2．5mmol／LandExTaqDNApolymerase1．0U．AndtheoptimalannealingtemperatureWas50degrees℃．3、Preservationusinglaidafoundation6prim

6、erpairsWerescreenedoutfrom100SRAPprimerpaircombinationsbasedontheirstableamplification，clearbandingpatternsandgoodpolymorphismbyutilizingtheSRAPreactionsystem．eachprimercombinationproduced4—11poly-morphicloci；PercentofPolylnorphicloci54．16％·80．55％withallave

7、rageof65．4％．4、Sophorajaponicaprovenanceofgeneticdiversityindex：Totalnumberofallels(Na)Was1．88890f,effectivenumber(Ne)Was1．6527，Nei’Sgenediversity(均Was0．3691，Shannon’SInformationindex(Dwas0．5359，Itwasbasicallyidenticalthatgeneticlevelin32provenanceeffectiven

8、umberofeachindex．Thesignificancedifferencedidnotexistingeneticdiversityof32provenancebyanalysis．5、GeneticdiversityindexofprovenancesofSophorajaponica：Ht=O．3691，Hs20．3338，Gst=-0．0958，Thepercentageofgene