携带线粒体CO1_tRNASer(UCN)7444G_A突变和12S rRNA 1555A_G突变的非综合征型耳聋家系的线粒体功能研究

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1、分类号：Q3单位代码：10343学号：151004727硕士学位论文Ser(UCN)论文题目:携带线粒体CO1/tRNA7444G>A突变和12SrRNA1555A>G突变的非综合征型耳聋家系的线粒体功能研究研究生姓名：时雯雯学科专业：免疫学类型：学术型指导教师：管敏鑫二O一八年五月Ser(UCN)论文题目：携带线粒体CO1/tRNA7444G>A突变和12SrRNA1555A>G突变的非综合征型耳聋家系的线粒体功能研究答辩委员会主席：答辩委员会成员：论文答辩日期：目录中文摘要...........

2、..................................................................................1ABSTRACT.........................................................................................3第一章绪论..................................................................

3、......................5第二章实验材料与方法....................................................................72.1实验对象.................................................................................72.2研究内容......................................................

4、...........................72.3实验材料................................................................................72.3.1主要的实验试剂..........................................................72.3.2主要的实验仪器.....................................................

5、.....92.4细胞培养及细胞株的建立...................................................112.4.1贴壁细胞的培养.........................................................112.4.2细胞冻存....................................................................122.4.3细胞复苏....................

6、................................................122.4.4血小板融合细胞株的构建与鉴定............................122.5线粒体DNA突变与拷贝数分析........................................142.5.1外周血和细胞中DNA的提取.................................142.5.2DNA纯度的鉴定与保存.........................

7、..................152.5.3DNA序列的测定与分析...........................................152.5.4荧光定量PCR............................................................172.5.5PCR-RFLP鉴定突变位点........................................192.6线粒体tRNA表达水平的检测...................

8、......................202.6.1线粒体的提取............................................................202.6.2线粒体中RNA的提取.............................................212.6.3RNA纯度的鉴定与保存...........................................222.6.4NorthernBlo