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1、生理学报ActaPhysiologicaSinica,April25,2008,60(2):249-253249http://www.actaps.com.cn研究论文低氧预适应小鼠皮层Bcl-2和Caspase-3的表达变化张颜波1，吕国蔚2,*，杨明峰1，牛敬忠1，孙保亮11泰山医学院附属医院神经内科，泰安271000；2首都医科大学低氧医学研究所，北京100069摘要：本文旨在探讨小鼠皮层Bcl-2和Caspase-3在低氧预适应脑保护中的作用。将Blb/c近交系小鼠随机分为对照组、低氧组和低氧预适
2、应组，用免疫荧光和激光共聚焦显微镜等技术测定皮层顶叶Bcl-2和Caspase-3表达荧光强度和阳性细胞计数。结果显示，低氧组和低氧预适应组Bcl-2表达均显著高于对照组，低氧预适应组又显著高于低氧组。低氧组和低氧预适应组Caspase-3表达均显著高于对照组，但低氧预适应组显著低于低氧组。结果表明，低氧预适应过程中，小鼠皮层脑区通过Bcl-2高表达和Caspase-3低表达抵御皮层细胞凋亡，从而参与脑保护机制。关键词：低氧预适应；Bcl-2；Caspase-3；凋亡；皮层；小鼠中图分类号：R364.2C
3、hangesinBcl-2andCaspase-3expressionsincortexofhypoxicpreconditioningmiceZHANGYan-Bo1,LUGuo-Wei2,*,YANGMing-Feng1,NIUJing-Zhong1,SUNBao-Liang11DepartmentofNeurology,AffiliatedHospitalofTaishanMedicalCollege,Tai’an271000,China;2InstituteforHypoxiaMedicine,C
4、apitalMedicalUniversity,Beijing100069,ChinaAbstract:ThepurposeofthepresentstudywastoexploretherolesofBcl-2andCaspase-3inmousecortexinhypoxicpreconditioning.Blb/cmicewererandomlydividedintothreegroups:controlgroup,hypoxicgroupandhypoxicpreconditioninggroup
5、.FluorescenceintensityofBcl-2andCaspase-3wasobservedandnumberofpositivecellswascountedinparietalcortexbyimmunofluorescenceandconfocallaserscanningmicroscope.FluorescenceintensityofBcl-2inthenormalgroup,hypoxicgroupandhypoxicpreconditioninggroupwas6.2±1.7,
6、68.5±13.1,180.6±34.8,respectively,andnumberofBcl-2-positivecellswas18.5±4.9,52.3±10.5,150.8±24.7,respectively.FluorescenceintensityofCaspase-3inthecontrolgroup,hypoxicgroupandhypoxicpreconditioninggroupwas8.6±2.0,40.2±8.2,26.4±6.1,respectively,andnumberof
7、Caspase-3-positivecellsofwas4.3±1.2,63.6±12.5,45.7±9.8,respectively.TheresultsshowedthattheexpressionsofBcl-2inbothhypoxicgroupandhypoxicpreconditioninggroupweresignificantlyhigherthanthatinthecontrolgroup;andtheexpressionofBcl-2inhypoxicpreconditioninggr
8、oupwasevenhigherthanthatinhypoxicgroup.TheexpressionsofCaspase-3inhypoxicgroupandhypoxicpreconditioninggroupwerealsosignificantlyhigherthanthatinthecontrolgroup;whereastheexpressionofCaspase-3inhypoxicpreconditionin
