生物化学实验ppt培训课件

《生物化学实验ppt培训课件》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、ExperimentsinBiochemistry生物化学实验DepartmentofBiochemistryBiochemistry&MolecularBiologyExp3ElectrophoresisofserumproteinsoncelluloseacetatemembranePurpose:TomastertheprincipleofelectrophoresisTolearntoseparateserumproteinsbycelluloseacetateelectrophoresisPri

2、nciple:Electrophoresis（电泳）___+Serumproteins:Albuminα1-globinα2-globinβ-globinγ-globinMaterialsandmethods:Materials/reagents:Celluloseacetate(2×8cm)Electrophoresisbuffer(电泳缓冲液）Proteinstainsolution（染色液）destainingsolution（漂洗液/脱色液）Methods:Preparingforthecellu

3、loseacetateLoadingsamples(serumorγ-globin)Electrophoresis(110V×45~60min)Staining:2-3minDestaining:ResultsMakecertaineverybandoncellulosecorrespondingtowhichkindofserumprotein+_????bandsoncelluloseofserumprotein????ThinkingandDiscussion1.Page92.Analyzethep

4、robablyreasonsaccordingtoyourselfresults.