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ID:33821428
大小:3.60 MB
页数:35页
时间:2019-03-01
《电针对mcao大鼠脑皮质臂板蛋白3a和轴突生长导向因子-1表达的影响》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、万方数据重庆医科大学硕士研究生学位论文sema3a,主要集中在细胞质阳性表达。Westemblot检测结果显示,与正常组相比,模型组Ntnl蛋白的表达水平在脑梗死后ldN]开始明显上升(P2、d时达高峰(P<0.01),14d时仍高于基础水平(P<0.01)。同时相点电针组与模型组相[Zsema3a均呈现低表达,1d、3d_;}1]7d、14d时有统计学差异(P<0.05,P<0。01),峰值同样在7d时出现。Westernblot检测结果与免疫组化分析结果基本相符。7d、14d时电针组与模型组相LLmNSS评分存在统计学差异(P<0.05),14d时电针组与模型组相LkiNF200的表达量存在统计学差异(P<0.05)。结论局灶性脑梗死后大鼠皮质Ntnl与sema3a表达明显上调,给予电针干预后可提高Nml的表达并抑制sem3、a3a的表达,促进轴突的再生与修复,这可能是电针促进脑梗死后神经功能恢复的机制之一。关键词:脑缺血再灌注;电针;轴突生长导向因子一l;臂板蛋白3a万方数据重庆医科大学硕士研究生学位论文EFFECToFELECTRO.ACUPUNCTURE0NEXPRESSIONOFNETRIN一1ANDSEM睑PHoRIN3AINRATCoRTEXAFTERMIDDLECEREBRALOCCLUSIoNABSTRACTAbstractObjectiveToobservetheexpressionofnetrin-1(Ntnl)andsemaphorin一4、3a(sema3a)inthecortexofratsaftermiddlecerebralocclusion(MCAO),anddeterminetheeffectofelectro—acupuncture(EA)interventionontheirexpression,SOastoexploretherolesofNtnlandsema3ainneuralplasticityaftercerebralinfarction.MethodsAtotalof135maleSpragueDawley(SD)ratswererandomlyd5、ividedintocontrolgroup(n215),modelgroup(n560)andEAgroupfn=60,intheacupuncturepointsPC6andST36,80to100Hz,1to3mA,1to3V,for30min,performedin90minafteranesthesiarecovery),andfurtherassignedinto4timepoint,thatis,inld,3d,7dand14daftermodelestablishment.Modifiedmodifiedneurologi6、cseverityscores(mNSS)werecarriedouttoevaluateneurologicinjuryateverytimepoint.ImmtmohistochemicalassaywasusedtodetecttheexpressionanddistilbutionofNtnl,sema3aandNF200inthecorticalischemicregion.WesternblouingwasemployedtodetecttheexpressionofNtnlandsema3aintheaffectedcort7、ex.万方数据重庆医科大学硕士研究生学位论文ResultsImmunohistochemicalresultsshowedthattheratbraincortexexpressedNtnlandsema3aateverytimepoint,andtheymainlylocatedinthecytoplasm.WesternblottingindicatedthatthemodelgrouphadhigherexpressionofNtnlat1d(P8、akat7d(P<0.01),andmaintainedstillhigherat14dthannormallevels(P<0.01).Comparedwiththecontrolgroup
2、d时达高峰(P<0.01),14d时仍高于基础水平(P<0.01)。同时相点电针组与模型组相[Zsema3a均呈现低表达,1d、3d_;}1]7d、14d时有统计学差异(P<0.05,P<0。01),峰值同样在7d时出现。Westernblot检测结果与免疫组化分析结果基本相符。7d、14d时电针组与模型组相LLmNSS评分存在统计学差异(P<0.05),14d时电针组与模型组相LkiNF200的表达量存在统计学差异(P<0.05)。结论局灶性脑梗死后大鼠皮质Ntnl与sema3a表达明显上调,给予电针干预后可提高Nml的表达并抑制sem
3、a3a的表达,促进轴突的再生与修复,这可能是电针促进脑梗死后神经功能恢复的机制之一。关键词:脑缺血再灌注;电针;轴突生长导向因子一l;臂板蛋白3a万方数据重庆医科大学硕士研究生学位论文EFFECToFELECTRO.ACUPUNCTURE0NEXPRESSIONOFNETRIN一1ANDSEM睑PHoRIN3AINRATCoRTEXAFTERMIDDLECEREBRALOCCLUSIoNABSTRACTAbstractObjectiveToobservetheexpressionofnetrin-1(Ntnl)andsemaphorin一
4、3a(sema3a)inthecortexofratsaftermiddlecerebralocclusion(MCAO),anddeterminetheeffectofelectro—acupuncture(EA)interventionontheirexpression,SOastoexploretherolesofNtnlandsema3ainneuralplasticityaftercerebralinfarction.MethodsAtotalof135maleSpragueDawley(SD)ratswererandomlyd
5、ividedintocontrolgroup(n215),modelgroup(n560)andEAgroupfn=60,intheacupuncturepointsPC6andST36,80to100Hz,1to3mA,1to3V,for30min,performedin90minafteranesthesiarecovery),andfurtherassignedinto4timepoint,thatis,inld,3d,7dand14daftermodelestablishment.Modifiedmodifiedneurologi
6、cseverityscores(mNSS)werecarriedouttoevaluateneurologicinjuryateverytimepoint.ImmtmohistochemicalassaywasusedtodetecttheexpressionanddistilbutionofNtnl,sema3aandNF200inthecorticalischemicregion.WesternblouingwasemployedtodetecttheexpressionofNtnlandsema3aintheaffectedcort
7、ex.万方数据重庆医科大学硕士研究生学位论文ResultsImmunohistochemicalresultsshowedthattheratbraincortexexpressedNtnlandsema3aateverytimepoint,andtheymainlylocatedinthecytoplasm.WesternblottingindicatedthatthemodelgrouphadhigherexpressionofNtnlat1d(P8、akat7d(P<0.01),andmaintainedstillhigherat14dthannormallevels(P<0.01).Comparedwiththecontrolgroup
8、akat7d(P<0.01),andmaintainedstillhigherat14dthannormallevels(P<0.01).Comparedwiththecontrolgroup
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