中华绒螯蟹(eriocheir+sinensis)acrosin蛋白的定位和功能的初步探究

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1、末期GMl30信号在成熟精细胞中项体的残留。顶体作为一个囊泡型细胞器，在中华绒螯蟹的精子形成过程中，很可能便是起源于同是囊泡性的高尔基体。关键词：acrosin，精卵作用，卵透明带，高尔基体，精子形成，水解酶活性，顶体AbstractChineseIIlittencrab，Eriocheirsinensis，isanimportantkindofcrabwithhi曲economicalandnutritionalvalue．Itprovideagoodmodelfortheresearchinfieldslikedevelopmentalbiology，evolutionarybiol

2、ogyandreproductivebiology．ThecurrentcultivationofChinesemittencrabinChinawitnesstheincreaseinproductionaswellasthedeclineinqualityandpromiscuityinspecies．Tosolvethisproblem，pivotalistopayattentiononthereproductiveresearchofEsinensis．Spermatogenesisandfertilizaitonaretwoimportantissuesinreproducti

3、vebiology．Themostseriousobscurityinfertilizationisthecomplexityintherecognization，penetrationandfusionofgametes．InE．sinensis，thespermatogenesis，includingsperiogenesis，isjustascomplexasthatinmammals．Acrosinisanimportantserineproteaseinmammalianfertilizaiton．Itisofgreatsignificancetoexplorethefunct

4、ionofacrosinininferioranimalslikecrab，SOastounveilthecomplexityoftheirreproduction．Thiscurrentproject，takingthespermatogenesisincrabforthemodel，contributetoexpandourunderstandingofthemechanismunderlyingtheirreproduction．Weclonedthefull．1en9曲eDNAofacrosininChinesemittencrab，andemploytechniquesincl

5、udingwesternblottingandimmunofluorescence，toinvestigatetheimportantroleofacrosininthespermatogenesisandfertilizationofE．sinensis．A．TheexpressionspecificityofacroisninE．sinensis．Initially，theeDNAcloningofacrosininE．sinensisdemonstrateitsexpression．Moreover，theRT—PCRandwesternblowingresultsindicate

6、theexpressionspecificityofacrosinintestis，onthelevelofmRNAandproteinrelatively．Besides，qRT—PCRresultrevealstheelevatingexpressionofacrosininmaturecrabincomparisontotheyounginduviduals．Thisalsoimplicatestherequirementofacrosinforacrabindividual，andthesignificanceofacrosintoreproduction．B．Thepredic

7、tionoftertiarystructureofE．sinensisacrosin．ThepredictionoftertiarystructureillustratesthezymogendomainandcatalyticdomainofE．sinensisacrosinprotdYl．TherelativefunctionofthesetWOdomains，mayrevealtheroleofacrosinduringfer