xrcc4在乳腺癌组织中的表达与其临床意义

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1、XRCC4在乳腺癌组织中的表达及其临床意义ABSTRACTBackgroundBreastcanceristhemostprevalentcancerinwomenaroundtheworld，becomingthefastestgrowingcancerinurbanmortality．DNAdoublestrandbreaks(DSBs)，whichhappenatrelativeorneighboringbasesinbotllstrand,isthecrucialpointamongthosepath

2、ogenesisofbreastcancer．Asoneofthemostsevereinjuriesofcells，DSBscallcausedeletionandrearrangementofgeneticinformationandsubsequentlyresultinapoptosisormutation．DSBsrepairinvolvestwosignalingpathway：non-homologousendjoining(NHEJ)andhomologousrecombinational(I

3、-IR)．NHEJ，whichisthemainlyrepairmodeineukaryotes，mcanswi也thehelpofrepairablecomponent,DNAattheendsofthefractureseormectdirectlyindependentoftheDNAhomology．What'smore，X—myrepaircrosscomplementingprotein4(XRCC4)playinganimportantroleinNHEJpathway,isthevitalho

4、usekeepinggeneaboutmaintainingthestabilityofgenome．Previousstudieshaveconfirmedthat—singlenucleotidepolymorphism(SHIP)inXRCC4isrelevanttotheriskofbreastcancer,however,lessresearcheshavebeendoneaboutproteinexpressioninbreastcancertissues．objectiveDepended011

5、variousclinicaldatacollectionandtissuemicroarray,throughstudyingonXRCC4expressioninrespectivelybreastCanCertissuesandadjacentnormaltissues，andsubsequentlycombinetheresultswithclinicopathologicalparametersandoverallsurvival(OS)，thisresearchisaimedatinvestiga

6、tingwhetherXRCC4Callbetreatedasbreastpredictorandprognosticfactor,andfurtheranalysisingtheexpressionofXRCC4indifferentsubtypesofbreastcancer,andguidingclinicaltherapy．MethodsCollect284surgicalspecimensfrombreastcancerpatientsand77specimensaboutadjacentnorma

7、ltissueinthem；thencollect132surgicalspecimensfromtriplenegativebreastcancerpatientsand90specimensaboutadjacentnormaltissueinthem,aftermakingthemintotissuemicroarray,wecallinvestigatetheexpressionandclinicalsignificanceofXRCC4inrespectivelybreastcancertissue

8、s，triplenegativebreastcancertissuesandadjacentnormaltissuesbyusingimmunohistoehemicalmethod(Envisionmethod)．SPSS19．0so做vareapplicationsofthedatawillbeused．3．第二军医大学硕士学位论文．4一forstatistical．analysis．P<0．0