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ID:33007760
大小:2.13 MB
页数:50页
时间:2019-02-19
《米诺环素对大鼠脑缺血再灌注后nf-κb、iκbα和tnf-α表达的影响》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、AbstractneuroprotectivemechanismofMinocycline.Materialandmethods72healthyadultS-DratswererandomlydividedintotheSham—operatedgroup(Sham),ischemia-reperfusiongroup(IR)andMCtreatmentgroup(MT).ThemodelofIntraluminalThreadOcclusionModelwasestablishedaccordingtoZeaLongxsmethodafteraadvisableimprovement.T
2、heoperationofShamgroupWastreatedjustthesameastheothertwogroupsbesideswithouttheinsertionthread.MinocyclineWasdeliveredbyintragastricadministrationafterreperfusion(firstdoseWas45mg/kg,others22.5mg/kgevery12h),andbotllTheShamgroupandIRgroupweredeliveredphysiologicalsalineatthesametimepointuntiltheywe
3、resacrificed.ThebrainhistopathologyWasobservedat48hafterreperfusionbyH—Estaining.WedetectedneuronapoptosisbyTUNELtoevaluatetheischemia-reperfusioninjuryandtheneuroprotectiveeffectofminocyeline.TheexpressionofIrBa、NF—xBp65andTNF-awerefurtherdetectedbyimmunohistochemistrytoobservethevariationandtheef
4、fectsofminocycline.Results(1)ComparedwiththeIRgroup,thepathologicalchangeofbraintissueWasobviouslyrelievedaftercerebralischemia-reperfusion48h;(2)ComparedwiththeIRgroup,theneuronapoptosiswassignificantlydecreasedintheMTgroupaftercerebralischemia-reperfusion48h;(3)TheexpressionofNF-rBp65positivecell
5、shasincreasedsignificantlyafterreperfusion6handapproachthemaxon24h,thenithasslightlydecreaseon48h.TheexpressionofTNF·apositivecellshasincreasedsignificantlyafterreperfusion6handapproachthemaxon24h,thenitstillkeepahighlevelon48h.Ontheaccordingtimepoint,thepositivecellnumbersofNF-rd3p65orTNF-ainIRgro
6、upweresignificantlyhigherthantheShamgroup(P7、ons(1)nleexpressionofIrBaWaSdecreasedbutNF一加p65Wasover-expressedinratsafterFocalCerebralIschemia-Reperfusion.(2)MCcanincreaseTheexpressionofIxBabutdeceasetheexpressionofNF-加p65andTNF一%MCplayaneuroprotective
7、ons(1)nleexpressionofIrBaWaSdecreasedbutNF一加p65Wasover-expressedinratsafterFocalCerebralIschemia-Reperfusion.(2)MCcanincreaseTheexpressionofIxBabutdeceasetheexpressionofNF-加p65andTNF一%MCplayaneuroprotective
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