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ID:32050391
大小:1.81 MB
页数:66页
时间:2019-01-31
《粉尘螨变应原derf2、derf5基因的克隆表达与免疫学初步分析》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、粉尘螨变应原IX-rt2、D℃m基因的克隆表达及免疫学的初步研究Cloning,ExpressionandPrimaryImmunologicStudyofDerf2,Derf5twoAllergensfromDermatophagoidesFarinaeSubject:PathogenicbiologyMaster:ZhanghaoAdvisor:ZhengXiaoyingDepartmentofParasitology,ZhongshanSchoolofMedicine,SunYat—SenUniversity,Guangzhou,510080AbstractWitht
2、hechangeoflifestyleandpollutionofenvimnn舱nt,theprevalencerateofallergicdiseaseskeepsrising.Allergicdiseasesnotonlydoharmtopeoplehealth,butalsobringgreateconomicburdentofamiliesandsociety.Allergicdiseasesinducedbydustmitesarcthemostcommonallergies.DustmitesarethoughttObemajorcontentsofhouse
3、allergensandthestrongestallergenssincethesixtiesofthe20nIcentury.Mostasthma,allergicrhinitisandatopicdermatitisarerelatedtodustmites.Allergicdiseasesdamagepatientslhealthheavily,however,thereisstillnosatisfieddiagnosticagentsandtherapies.Inourcountry,thecrudeextractfromdustmitesisalwaysado
4、ptedfortreatment,whileitincludesmanykindsofallergicandnonallergiccontentsandisdifficulttoIV粉尘螨变应原Derf2、Derf5肇园的克隆表达及免疫学的初步研究standardme.Sideeffectsmayresultsfromimmunotherapywithcrudemiteextract,suchasallergyandasthma.Furthermore,immunotherapywithcrudemiteextractisdefectivebecausetheallerge
5、nssupplyisdifficult,theallergicproteinsarehardtostandardizeandpurify,etc.Therefore,itisveryimportanttostudyanddevelopnewsaferallergenvaccineswimhigh-effect.ObjeetiveIsolationandpurificationofthegroupIIallergenandgroupVallergenfromthedustmites,DermatophagoidesFarinaeforimmunotherapyarediffi
6、cult.InordertoobtainabundantrecombinantallergenproteinDerf2andDerf5forthefurtherstudyOlldevelopmentofnovelhomemadeanti—allergydrugsandproducepurifiedstandardizedrecombinantallergenproteinforimmunotherapy,theprokaryoticexpressionsystemwasestablishedinattempttoefficientexpressrecombinantalle
7、rgenproteinDerf2andDerf5.MethodsThedustmites,D.farinaefromGuangZhouweresettleddownandreproducedinlargescaleinlaboratory.PairsofprimersweredesignedaccordingtOthepublishedDerf2andDerf5sequences.Theopenreadingframe(ORE)ofDerf2andDerf5geneswereamplifiedbyRT-PCRaft
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