rankrankl和骨保护素在类风湿关节炎患者外周血和滑液中表达的研究

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1、维普资讯http://www.cqvip.com华风湿病学杂志2005年7月第9卷箍!塑tn』』Y!!!：!·4l7·RANKRANKL和骨保护素在类风湿关节炎患者外周血和滑液中表达的研究尚鹏朱平樊春梅王彦宏【摘要】目的研究骨保护素(OPG)和核因子KB受体活化因子(RANK)及其配体(RANKL)在类风湿关节炎fRA)患者外周血和滑液中的表达，探讨RANKL、RANK和OPG在RA骨破坏中的意义。方法收集活动期RA患者治疗前后外周血、滑液和健康对照，以间接免疫荧光标记，流式细胞术检测RANK、RANKL和OPG在外周血和滑液中CD3~T淋巴细胞、中性

2、粒细胞和CD14~~0-核细胞的表达率及平均荧光强度。结果①RA组治疗前后外周血RANK、RANKL和OPG在CD3~T淋巴细胞、中性粒细胞和CD14单核细胞上的表达率差异无统计学意义(P>O．01)；RANK的表达强度治疗后高于治疗前(P<0．01)。②RA组外周血CD3q"淋巴细胞上RANKL及RANK的表达率和表达强度明显低于滑液，OPG的表达则高于滑液(P(0．01)，RANKL／OPG比率明显增高(P

3、下调OPG表达参与RA破骨细胞的分化和骨损伤的调节机制。【关键词】关节炎，类风湿；滑液；NF_KB；骨保护素ExpressionofRANkRANKLandosteoprotegerinoilcellsinperipheralbloodandjointfluidofrheuma-tIidarthritissHANGPenZHUPinFANChan—mei．WANGYnn—hong．DepartmentofClinicalIm—munology,XijingHospital,7eFourthMilitaryMedicalUniversity,XiⅡn71

4、0032．ChinaCorrespondingauthor：ZHUPing,Entail：zhuping@fmmu．edu．cn【Abstract】0bjectiveToinvestigatetheexpressionofreceptoractivatorofNF-KB(RANK)，receptoractivatorofNF-KBligand(RANKL)andosteoprotegerin(OPG)onmonocytes，lymphocytesandneutrophilsinperipheralbloodandsynovialfluidobtaine

5、dfromrheumatoidarthritisfRA)patients．anddiscusstheroleofRANK，RANKLandOPGinbonedestructionofRA．MethodsTheexpressionrateandintensityofRANK．RANKLandOPGonlymphoeytes．neutrophilsandmonocytesinperipheralbloodbeforeandaftertreatmentandsynovialfluidbeforetreatmentofl0activeRAaswellasinp

6、eripheralbloodof10normalhealthycontrolswerestudiedbyindirectimmunfluoresceneeflowcytometry．Results①TheexpressionrateofRANKLRANKandOPGinRAbeforeandaftertreatmenthadnosignificantdifference(尸>0．01)：buttheintensityofRANKexpressioninperipheralbloodaftertreatmentwashigherthanthatofbef

7、ore(P<0．01)：②0nRACD3cells．theexpressionrateandintensityofRANKandRANKLinperipheralbloodwerelowerthansynovialfluidwhereastheOPGexpressionwashigher．TheratioofRANKL／OPGheightenedmarkedly(P<0．01)．Conclu-sionThechangeofintensityofRANKsuggeststhatitmaybeinvolvedinthedevelopmentandprogn

8、osisofRAinflammation，andactivatedCD3cellsmaypla