ra序贯诱导小鼠胚胎干细胞向神经细胞分化及对4.1蛋白表达的影响

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1、郑州大学2008届硕t..学位论文中文摘要RA序贯诱导组之间无显著性差异(尸>0.05)。W七stem七lot检测显示:未分化ESCs中表达4.IN和4.IB蛋白，诱导分化后两者表达量都增加。结论1.RA序贯诱导法可在ESCs分化后第7d获得神经元样细胞，较4一/4+诱导法提前3d，且两者的NSE和GFAP阳性细胞率无差异，因此RA序贯诱导法可作为经典的4一/4+诱导法的替代方法。2.4.IN和4.IB蛋白的表达量随着ESCs分化为神经细胞而增加。关键词:胚胎干细胞;分化:维甲酸;神经细胞;4.1蛋白郑州人学2008届硕l研究生毕业论文英文摘要R

2、A一treatedSequentialMethodIndueedEmbryonieStemCellsDifferentiationintoNeuralCellsandtheExPressionChangeofProtein4.1aftertheInduetionPostgr时uateJiangYuanyUanTutorXingYingDeP良rtmentofPhysiology，theBasieMedicalCollegeofZhengZhouUniversityZhengZhou，Henan，450052AbstraCtEmbryonieste

3、mcells(ESCs)whiehderivedfromtheinnereellmass(ICM)ofblastoeyst一stageembryoshavetheabilitytoProliferateindefinitelyinvitroandthePluriPoteneytodifferentiateinto’anyeellt冲ederivedfromeetoderm，mesodermorendoderm·Lot景ofreportsindicatedthatESCseouldbedireetionallydifl触rentiatedinton

4、euraleellsinsPeeifieindueedsystemwhiehProvidedPraetiealevideneesforthetransPlantationofESCstotreateenternervoussystemdiseases.Sofar，themethodsusedtodirectionallyindueeESCsdifferentiationintoneuraleellsinelude4一/4+assay，five一stePmethod，SDIA(astromalcell一derivedindueingaetivity

5、)，adherentmonoculture，genemethods，and50on.4一/4+assay15themostclassiealmethod.However，ESCsmustformemb理oniebodies(EB)beforeinduetedbyRAwhiehmakesitdiffieulttocontrolandanalyZetheProeessofinduetion.Moreover’thismethodrequireslongculturetime，comPlieatedmaniPulationandstricteultUr

6、eeonditionineludingeelldensity，nutritionandequiPments.SearchingforanewinduetionmethodtoshortentheinduetionPeriodandsimPlifymaniPulationwillimProvethestudyonESCseultureanddifl笼rentiationinvitro.It15uneertainaboutthemechanismofESCsdifl七rentiationintoneuralcellsPresently.Manyres

7、earehersfoundthattheexPressionofsomeProteininereased(i.e.PKC6andPKC。)ordecreased(i，e.notchl)duringneuraleellsdifl七rentiation.StudyaboutratPC12eelldifferentiationandmouseeerebellumneurondeveloPmentrevealedthattheIV丫郑州大学2008届硕!:研究生毕业论文英文摘要exPressionamount，altemativesPlieingandl

8、ocationofProtein4.1changedaiongwiththedifferentiationofneuraleells.B