咪喹莫特对小鼠哮喘模型气道炎症和stat6表达的影响

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1、咪喹莫特对小鼠哮喘模型气道炎症和STAT6表达的影响作者：金淑贤，殷凯生，卞涛，陈子庆【关键词】哮喘EffectsofimiquimodonairmationandSTAT6expressioninmousemodelsofasthma　　【Abstract】AIM:ToobservetheeffectsofaerosolimiquimodontheantigeninducedairmationandtheexpressionsofTh2typechemokinesEotaxin,macropha

2、gederivedchemokine(MDC)andthymusandactivationregulatedchemokine(TARC)inmousemodelsofasthma.METHODS:Imiquimodanddexamethasoneinisteredtoimmunizedmicebeforeantigenchallenge.At24hafterthefinalOVAinhalation,theleftsuperiorlungtissuemationberofcellsandclas

3、sifythem.Theexpressionsofsignaltransducersandactivatorsoftranscription1(STAT1)andsignaltransducersandactivatorsoftranscription6(STAT6)inlungsmunohistochemistryandRNAexpressionsofSTAT1,STAT6,IL4,eotaxinandIFNγinlungsinedbyreversetranscriptasepolymerase

4、chainreaction.RESULTS:①Imiquimodanddexamethasoneattenuatedtheairmationofasthmaticmice.②Imiquimoddecreasedthetotolcellcounts,EOSandlymphocytecountsinBALf.③TheexpressionsofSTAT1andSTAT6ainlyonthebronchialepithelium.STAT1andSTAT6protEinsandmRNAlevelsincr

5、easedinlungtissuesinasthmagroup.ImiquimodsignificantlyreducedSTAT1andSTAT6protEInandmRNAexpression.DexamethasonesignificantlyreducedthelevelsofSTAT6proteinandmRNA,butfailedtoinhibittheexpressionofSTAT1.④ImiquimodmodulatedtheTh1/Th2responsebyenhancingT

6、h1cytokineIFNγmRNAandinhibitingtheTh2cytokineIL4mRNAexpressioninlungtissues.CONCLUSION:ImiquimodaerosolinhalationmayinhibitantigeninducedairmationanddecreasetheoverexpressionofSTAT6proteinandmRNAinasthmaticmousebuthasnoinfluenceonSTAT1expression.　　【Ke

7、ya;signaltransducersandactivatoroftranscription1;signaltransducersandactivatoroftranscription6;imiquimod　　【摘要】目的：观察咪喹莫特对小鼠哮喘模型气道炎症和肺组织信号转导与转录激活因子1（STAT1）和STAT6表达的影响.方法：建立哮喘模型，自14d时雾化吸入OVA前0.5h，干预组分别雾化吸入咪喹莫特30min及ip地塞米松.OVA雾化结束后24h取左上叶肺组织做HE染色观察肺组织炎症改变

8、；收集肺泡灌洗液进行细胞计数和分类；用免疫组化和RNA表达水平.结果：①HE染色示地塞米松组和咪喹莫特组小鼠肺组织炎症程度较哮喘小鼠减轻.②哮喘组小鼠BALF中细胞总数及各种炎症细胞较正常组显著增加，咪喹莫特治疗组嗜酸性粒细胞、淋巴细胞比哮喘组明显减少(P<0.01).③STAT1和STAT6均在气道上皮细胞表达，哮喘组肺组织STAT1和STAT6表达较正常组增强，咪喹莫特组STAT1表达与哮喘组无明显区别，较正常组增加.④哮喘组小鼠肺组织STAT1,STAT6,eotaxi