绣球菌多糖提取、分离纯化及生物活性研究

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1、AbtractSparassiscrispaisakindofediblefungi，withthefunctionalactivitiessuchasanticancer，anti-tumor，antivirus，toimprovemetabolism，decreasethebodybloodfatandbalancethebloodpressure，improvethehematopoieticfunctionandimmunorregulation.PolysaccharidesinSparassiscrispaisoneofthemostimportantlyactiveingred

2、ient．Thecontentofβ-glucangoesupto50%-60%inSparassiscrispapolysaccharides．ThepolysaccharideextractedfromSparassiscrispaposesasensationalcommercialpotential.Sofar，thecultivationandprocessingonSparassiscrispahasincreasedprogressly,whereasthestudyinthefieldpolysaccharideextraction,it’sproductdevelopmen

3、tandclinicalapplicationremainsinblank.Inthisthesis,thepolysaccharideextractionfromSparassiscrispawithcompoundenzyme,proteindetachingfromthecrudepolysaccharide,theantioxidationactivityandthestructuralidentificationofpolysaccharideextractedfromSparassiscrispawereimplementedtoprovideavailabletheorybas

4、isforfurtherdevelopmentandapplicationofSparassiscrispa．Enzymessuchascellulose，pectaseandpapainarecompoundedtoextractpolysaccharidefromSparassiscrispa．Theratioofthecompoundenzymesandtheenzymolysisparameterswereoptimizedthroughtheorthogonalexperimentandtheresponsesurfacemethodrespectively．Then,thedep

5、roteinization，dialysistreatment,columnchromatographytreatmentwereemployedtoseparateandpurifytheSparassiscrispapolysaccharide，Appraisaltheextracorporalantioxidationactivityofthedeproteinizedcomponent，uniformcomponentandcrudepolysaccharide；AnalysisedthepolysaccharidestructurethroughIRspectrumandNMRsp

6、ectrum．(1)TheextractionpolysaccharidefromSparassiscrispawithcompoundenzymesTheextractingconditionspolysaccharideformSparassiscrispawithcompoundenzymeswasoptimizedasfollows：cellulose0.6%，pectase0.4%andpapain0.6%，enzymolysispH4.16，time3.41h，temperature53.73℃，theratioofsolidIIIvsliquidwas1：15.63.Andun

7、derthisconditionthepolysaccharideextractionratewasupto14.3%．(2)ThedeproteintechnicsforSparassiscrispapolysaccharideEnzyme,calciumchloride,hydrochloricacid,TCAandsevagemethodswereusedrespectivelytoremovethep