体内构建骨软骨复合体修复骨软骨缺损的实验研究.doc

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1、体内构建骨软骨复合体修复骨软骨缺损的实验研究【摘要】探讨软骨组织工程方法修复骨软骨缺损理想的种子细胞和支架材料。［方法］体外诱导骨髓基质干细胞向软骨细胞定向分化，分别与PLGA支架复合培养，并模仿马赛克骨软骨移植术在犬关节骨软骨缺损深层置入MSC—支架复合体，浅层置入诱导培养后的MSC_支架复合体，紧密压配，体内构建骨软骨复合体，观察其修复的情况。［结果］16周实验组缺损区完全由透明软骨修复，与周围正常软骨完全融合，组织观察显示以软骨细胞修复为主，软骨下骨形成，潮线基本恢复；阴性对照组缺损区主要由纤维组织修复，部分由透明软骨修复，组织观察

2、显示以纤维细胞修复为主，混有部分软骨细胞；空白对照组完全由纤维组织修复。［结论］MSCs经向软骨细胞定向分化后复合PLGA支架材料，通过紧密压配方式与MSCs-PLGA支架在体内构建骨软骨复合体，可以有效修复骨软骨缺损。【关键词】骨髓基质干细胞马赛克移植术骨软骨复合体骨软骨缺损Abstract:［Objective］Toevaluatetheeffectoftreatingtheosteochondraldefectswithimplantedcellscaffoldcomposites,culturedMSCsasseedcellsan

3、dPLGAasscaffolds,andtoacquiredesirableseedcellsandscaffoldmaterials.[Method]BMSCswereinducedtodifferenciatiatedintochondrocytes,coculturedwithPLGAscaffoldrespectivelyinvitro,thenimplantedintoosteochondraldefectsoncaninemodelsbyusingtechniquesofmosaicplasty,inducedBMSCPLGA

4、scaffoldcompositesinthetopofthedefectandBMSCPLGAscaffoldcompositesinthebottomofthedefect,osteochondralcompositeswereconstructedinvivo,andrepairwasobservedwithnakedeyesandhistology.[Result]Atl6weeksaftertransplantationthedefectsofexpEirmentalgroupwerecoveredwithsemitranspa

5、rentsmoothwhitetissueandthemarginsbetweentherepairtissueandthesurroundingcartilagewerenotrecognized.Histologically,mostoftherepairtissuewasconsistedwithchondrocytes,maintainedthEIrthicknesstothefulldepthoftheoriginaldefects.Thesubchondralbonewaswellremodeled.Thetidemarkwa

6、sobserved.Thedefectsofpositivecontrolgroupwerecoveredwithrepairtissues,andpartialwereconformedwithoriginalcartilage.Therepairtissuewaspartlyfilledwithchondrocytes.However,thedefectsofnegativecontrolgroupwererepairedwithsoftfibroustissueswithoutluster,andanobviousboundaryb

7、etweenreparativeandoriginalcartilagewasseen.[Conclusion]MSCsPLGAscaffoldcomposites,constructedintoosteochondralcompositesbysuppressingcloselyinvivo,aretheidealmaterialsforrepairingcartilagedefects.Keywords:bonemesenchymalstemcells(MSCs)；mosaicplasty；osteochondralcomposite

8、s；osteochondraldefect关节软骨损伤是骨科临床的常见疾病，以往临床使用的治疗方法均难以迗到理想的要求。组织工程技术的迅速发展，为这一难题的解决提供了前所未有的机遇。本实验研究