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1、天祝白牦牛饲草致病菌分析沈慧1,余群力1,陈其元1,胡平2,权郁玲2(1.甘肃农业大学食品科学与工程学院,甘肃兰州;2.甘肃省疾病预防控制中心,甘肃兰州730070)摘要:以天祝白牦牛的饲草为对象,定量研究天祝县石门、炭山岭、松山、西大滩、抓喜秀龙5个地点的白牦牛饲草(夏秋牧草、冬春牧草)中志贺氏菌、金黄色葡萄球菌、病原性大肠艾希氏菌、沙门氏菌、小肠结肠炎耶尔森氏菌、单核细胞增生李斯特氏菌、空肠弯曲菌7种消化道致病菌污染情况,并分析白牦牛饲草料污染7种致病菌的主要原因。夏秋牧草中各种消化道致病菌检出率为志贺氏菌(41.24%)>金黄色葡萄球菌(12.19%)>沙门氏菌(7.32
2、%)>病原性大肠艾希氏菌(4.88%),而小肠结肠炎耶尔森氏菌、单核细胞增生李斯特菌、空肠弯曲菌未检出;冬春牧草中的检出率为志贺氏菌(10.25%)>金黄色葡萄球菌(7.69%),而病原性大肠艾希氏菌、沙门氏菌、小肠结肠炎耶尔森氏菌、单核细胞增生李斯特菌、空肠弯曲均未检出。5个采样点中,夏秋牧草中7种消化道致病菌总体污染程度为石门(100.00%)>炭山岭(62.5%)>松山(60.00%)>西大滩(14.28%)>抓喜秀龙(0);冬春牧草总体污染程度为炭山岭(33.33%)>石门(28.57%)、松山(25.00%)>西大滩(0)、抓喜秀龙(0)。致病菌污染夏秋季高于冬春季。
3、天祝白牦牛饲草已经存在轻度致病菌污染,主要是人类活动的增多造成的,对白牦牛肉及产品存在一定危害。关键词:白牦牛;饲草料;致病菌AnalysisonthePathogenoftheForageGrassofWhiteYakinTianZhuSHENHui1,YUQun-Li1,CHENQi-Yuan1,HUPing2,QUANYu-Ling2(1.CollegeofFoodScienceandEngineering,GansuAgricultureUniversity,730070;2.GansuDiseaseControllingandPrecautionCenter,Gans
4、u,Lanzhou730000,China)Abstract:TakingtheforagegrassofwhiteyakinTianzhu(Shimen,Tanshanling,Songshan,Westdatan,Zhuaxixiulong)astheobject,thesevenkindsofpathogenicgermsincludingShigella,Staphytococcusaureus,Salmonella,Yersiniaenterocolitica,Escherichiacoli,Listeriamonocytoyenes,Campylobacterjej
5、uniintheforagegrass(summer/autumnandwinter/spring)ofwhiteyakwerestudiedquantitatively,andanalysisedthemainpollutionreasonoftheforagegrass.Thepositiveratesof7kindsofpathogenicgermsinsummer/autumngrasswereShigella(41.24%)>Staphytococcusaureus(12.19%)>Salmonella(7.32%)>Escherichiacoli(4.88%),bu
6、tYersiniaenterocolitica,ListeriamonocytoyenesandCampylobacterjejunihadnotbeendetectived.Thepositiveratesinwinter/springgrasswereShigella(10.25%)>Staphytococcusaureus(7.69%),butSalmonella,Escherichiacoli,Yersiniaenterocolitica,ListeriamonocytoyenesandCampylobacterjejunihadnotbeendetectived.Th
7、epollutiondegreesof7kindsofpathogenicgermsinsummer/autumngrasswereShimen(100%)>Tanshanling(62.5%)>Songshan(60.00%)>(14.28%)>Zhuaxixiulong(0)andthepollutiondegreesinwinter/springgrasswereTanshanling(33.33%)>Shimen(28.57%)andSongshan(25.00%)>Westdata