treatment of traumatic brain injury in rats with transplantation of human amniotic cells

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1、TreatmentoftraumaticbraininjuryinratswithtransplantationofhumanamnioticcellsChineseMedicalJournal2006;119(21).1843—1845l843BriefreportTreatmentoftraumaticbraininjurytranSplantationofhumanamnioticinratswithcellsLUYi,HUIGuo-zhen,WUZhi-yuan,GUOLi-he,J!Xun-heandWUXinKeywords:braininjuries;amnion;transpl

2、antation;ratsTraumaticbraininjury(TBI),whichisanimportantIreasonofhumanmortalityandmorbidityinindustriaIizedcountries.stilIcannotbetreatedeffectively.Sincetheself-repaircapacityofbrainisIimited.cellulartransplantationinTBImaybeatherapeuticoption.Humanamnioticcells(HACs1fr0mfertilizedovumareabletodif

3、ferentiateintoalIthetissuesofthebody.TheycannotonlyexpressthemarkersofneuronaIandglialcells.butalsosynthesizeandreleasetheneurotrophicfactors.Moreover.transplantedHACsrarelyinduceimmunologicrejection.Inthisstudy.weinvestigatedwhetherHACculturesconditionedbytraumaticbraintissueextractscanacquiretheph

4、enotypeofneuron,andexaminedtheeffectsoftransplantedHACsontheaffectedhindlimbofratswithTBI.METHODSCelIseparationandcultureTheseparatedamnionwasdissectedintoseveralpieces,treatedwith0.25%trypsin(GibcoBRL)at37.Cfor15minutes,andrepeatedforthreetimes.ThentheisolatedHACswerecollectedbycentrifugation.After

5、cellularviabilitywasassessedbytrypanblue,HACswereculturedinRPMI1640medium(GibcoBRL)supplementedwith10%felaIbovineserum(FBS,GibcoBRL,USA).After48hoursthen0nadherentHACswerediscarded.CellfluorescencelabelingHoechst33342(Sigma,USA)wasmixedwithRPMI1640mediumtoyieldafinaIconcentrationof5pg/m1.Afterbeingl

6、abelledwithHoechst33342for30minutesat37.C,theHACswerecollectedandrinsedforthreetimesbyphosphate-bufferedsaline(PBS).AnimaImodelsAdultfemaleSprague-Dawleyrats(200—350g)wereobtainedfr0mShanghaiExperimentaIAnimalsInstituteofChineseAcademyofSciences.ImprovedFeeney'sweightdroptechniquewasmadebyallowinga2

7、0aweighttofalIontotherodfr0maheightof30cm.Aftertrauma.theboneflapwasimmediatelyreplacedandsealedwithdentaIcementTheratsofshamTBIgroupwereanesthetizedandreceivedcraniotomieswithoutweightdropiniuCelIcon