幽门螺杆菌全菌蛋白抗原壳聚糖微球体外释放特性

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1、www.CRTER.org刘微，等.幽门螺杆菌全菌蛋白抗原壳聚糖微球体外释放特性幽门螺杆菌全菌蛋白抗原壳聚糖微球体外释放特性*★刘微1,2，谢勇2，刘志军1PreparationandinvitroreleasecharacteristicsofchitosanmicrospheresloadingHelicobacterpyloriwholecellproteinantigenLiuWei1,2,XieYong2,LiuZhi-jun11DigestiveSystemDepartment,People'sHospitalofXinyu,

2、Xinyu338025,JiangxiProvince,China;2InstituteofDigestiveDisease,theFirstAffiliatedHospitalofNanchangUniversity,Nanchang330006,JiangxiProvince,ChinaLiuWei★,Master,Physician,DigestiveSystemDepartment,People'sHospitalofXinyu,Xinyu338025,JiangxiProvince,China;InstituteofDigest

3、iveDisease,theFirstAffiliatedHospitalofNanchangUniversity,Nanchang330006,JiangxiProvince,Chinabaibianfuliuwei@163.comCorrespondenceto:XieYong,Professor,Chiefphysician,InstituteofDigestiveDisease,theFirstAffiliatedHospitalofNanchangUniversity,Nanchang330006,JiangxiProvince

4、,Chinaxieyong_med@163.comSupportedby:theNationalNaturalScienceFoundationofChina,No.30460052*Received:2010-10-05Accepted:2011-03-12新余市人民医院消化内科，江西省新余市338025；2南昌大学第一附属医院消化疾病研究所，江西省消化疾病研究重点实验室，江西省南昌市330006刘微，男，1983年生，江西省新余市人，汉族，2010年南昌大学医学院毕业，硕士，医师，主要从事幽门螺杆菌感染免疫致病和免疫防治。baibia

5、nfuliuwei@163.com通讯作者：谢勇，教授，主任医师，南昌大学第一附属医院消化系病研究所，江西省消化疾病研究重点实验室，江西省南昌市330006xieyong_med@163.com中图分类号:R318文献标识码:B文章编号:1673-8225(2011)29-0000-04收稿日期：2010-10-05修回日期：2011-03-12(20101005006/M•W)AbstractBACKGROUND:ChitosanmicrospheresloadingHelicobacterpyloriwholecellproteina

6、ntigenpreparedbydifferentmethodshavedifferentthepackageratesandcontrolled-releaseeffects.OBJECTIVE:TopreparechitosanmicrospheresloadingHelicobacterpyloriwholecellproteinantigenandobservethereleasecharacteristicsinvitro.METHODS:Bertholdprecipitationmethodwasusedtopreparech

7、itosanmicrospheresandthepreparationwasoptimized;scanningelectronmicroscopeandparticlesizeanalyzerwereusedtoobservetheshapeandsizemeasurementofchitosanmicrospheres;theBCAProteinAssayKitwasusedtomeasuretheloadingefficiency,loadingcapacityandreleasingrateofmicrospheres.RESUL

8、TSANDCONCLUSION:Wechosethebestpreparationprogramofchitosanmicrospheresfrom32speciespreparationpr