黄瓜叶片衰老过程中脂滴的原位降解和液泡内降解

《黄瓜叶片衰老过程中脂滴的原位降解和液泡内降解》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、园艺学报，2016，43(10)：2021–2028.ActaHorticulturaeSinicadoi：10.16420/j.issn.0513-353x.2016-0266；http：//www.ahs.ac.cn2021黄瓜叶片衰老过程中脂滴的原位降解和液泡内降解*葛姝君，赵丹，周雪，孙晴，刘林（临沂大学药学院，山东临沂276005）摘要：对黄瓜不同发育阶段叶片中的脂滴进行超微观察，以期了解衰老叶内脂滴降解的场所。结果表明，衰老叶片中部分质体小球在叶绿体内降解，降解先从小球一侧开始，向另一侧推进。衰老叶片含有胞质脂滴，有些胞质脂滴紧贴叶绿体，显示其发生与叶绿体密切相关；有些胞质

2、脂滴与液泡密切接触，并向液泡内转运，进入液泡后发生降解。显然，黄瓜叶衰老不仅引起脂滴在液泡内降解，也引起脂滴在叶绿体内原位降解，证明液泡并不是脂滴降解的唯一场所。关键词：黄瓜；叶片；衰老；脂滴；原位降解；液泡内降解中图分类号：S642.2文献标志码：A文章编号：0513-353X（2016）10-2021-08CucumberLeafSenescenceCausesin-situandVacuolarDegradationofLipidDroplets*GEShu-jun，ZHAODan，ZHOUXue，SUNQing，andLIULin（CollegeofPharmacy，Linyi

3、University，Linyi，Shandong276005，China）Abstract：Leafsenescenceinducesup-regulatedexpressionoflipiddegradation-relatedgenes，andactivitiesoflipasesarefoundtobeenhancedinvacuoles.However，itisnotclearwhethervacuolesaretheonlysiteforthedegradationoflipiddroplets.Therefore，dynamicsoflipiddropletsincucu

4、mberleavesofdifferentdevelopmentalstageswereinvestigatedbymeansoftransmissionelectronmicroscopytounderstandthesitesforlipiddropletscausedbyleafsenescence.Someplastidiallipiddropletsinsenescentleavesweredemonstratedtoundergodegradationinsidechloroplasts.Degradationbeganfromonesideandthenexpandedt

5、otheother.Inthecytoplasmsomecytosoliclipiddropletswerefoundtobeattachedtothesurfaceofchloroplasts，indicatingthattheiroccurrencemighthaveacloserelationtochloroplasts.Somecytosoliclipiddropletsrevealedacloseassociationwithvacuoles，indicatingthattheywereonthepathwaytobetransportedintothevacuoles.Li

6、piddropletsunderwentdegradationaftertheyweretransportedintovacuoles.Apparently，cucumberleafsenescencecausedin-situdegradationofplastidiallipiddropletsandvacuolardegradationofcytosoliclipiddroplets.Theseobservationsprovedthatvacuolesarenottheonlysiteforthedegradationoflipiddroplets.Keywords：Cucum

7、issativus；leaf；senescence；lipiddroplet；in-situdegradation；vacuolardegradation收稿日期：2016–05–25；修回日期：2016–10–08基金项目：山东省自然科学基金项目（ZR2015HL115）*通信作者Authorforcorrespondence（E-mail：liulinlyu163@163.com）GeShu-jun，ZhaoDan，ZhouXue，SunQ