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1、Vol.43No.6研究报告稻/菰远缘杂交后代变异材料MITE类转座子mPing的激活111121时羽，韩康顺，孟令君，贾玉敏，周桂姣，李彦利*（1通化市农业科学研究院，吉林通化135007；2通化市种子公司，吉林通化134000）摘要：实验以日本引进粳稻品种松前为受体材料，以水稻的近缘属菰为供体，利用花粉管通道法导入受体水稻菰的基因组DNA。经过两个世代的培养，T1世代表型（株形、穗形、籽粒特征）变异不明显，但统计分析结果表明生育期及有效分蘖数与母本有显著差异。在T2世代发现明显的表型变异株，并且此变异后代类型丰富。随机选取22个表型变异株进行S
2、outhern杂交和转座子分析，发现MITE类转座子mPing发生了转座激活。根据日本晴的基因组序列设计了含有mPing的特异位点引物以及用TAIL-PCR方法检测到了T2世代中mPing的跳出及插入频繁。实验获得了水稻的优良变异后代，扩大了遗传资源，丰富了生物多样性。研究结果为远缘杂交在基因组进化和作物遗传改良中的可能作用提供了新的理论依据。关键词：远缘杂交；花粉管通道法；转座子激活；MITEs；mPing中图分类号：S511.035文献标志码：A文章编号：1673－6737（2013）06－0007－06MobilizationoftheAct
3、iveMITEsTransposonsmPinginVariantOffspringofRiceandZizanias'DistantHybridization111121SHIYu,HANKang-shun,MENGLing-jun,JIAYu-min,ZHOUGui-jiao,LIYan-li*(1TonghuaInstituteofAgricultureandScience,MeihekouJilin135007,China;2TonghuaCompanyofSeed,MeihekouJilin134000,China)Abstract：Th
4、isexperimentusedthevarietyofMatsumaewhichwasinducedfromJapanasrecipientmaterial,usedZizania.caducifloraofapproachingfringeofriceasthedonator,andusedtheaislemethodofpollentubeimportedthegenomeDNAofrecipientZizania.Afterthecultivationoftwogeneration,thevariationofphenotypeofT1ge
5、neration(theshapeofplantandspike,thefeatureofkernal)wasnotobvious,butthestatisticalanalysisresultsindicatedthatperiodofdurationandeffectivetilleringnumberhavesignificantdeviation.DiscoveredsignificantplantofphenotypicvariationintheT2generation,andthetypeofitsoffspringwasabunda
6、nt.Selected22extractionDNAfromvariantsofT2generationrandomly,anddidSouthernblotandtransposonanalysisintheconditionofchoosingphenotypicvariationplantrandomly,discoveredthatthemPingwhichpertainingtotransposonofMITETcategory.DevisedthespecificsiteprimerwhichincludingofmPingaccord
7、ingtothegenomesequenceofNipponbare.DetectedthatT2generationhadthedapofmPingbyusedthemethodofTAIL-PCR,thedapandinsertwasfrequent.Thisexperimentobtainedtheexcellentvariationoffspringofrice,thisenlargedinheritedresourceandenrichedbiodiversity.Theresultsofthisthesisofferedthenewth
8、eorybasesforthepossibleroleofdistancehybridizationinthegenome
