海岛棉gbmft基因的克隆及表达分析

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1、棉花学报CottonScience2014袁26渊3冤院197~203海岛棉2基因的克隆及表达分析1*1*1221**顾超袁郭丹丽袁张峰袁李雪源袁艾先涛袁黄先忠(1.石河子大学生命科学学院农业生物技术重点实验室袁新疆石河子832003曰2.新疆农业科学院经济作物研究所袁新疆乌鲁木齐830091)摘要院通过RT-PCR和RACE技术从新疆海岛棉品种新海14号中克隆得到了一个(1)类似基因袁命名2基因(GenBank登录号为KF739071)遥2基因的开放阅读框(ORF)为519bp袁编码172个氨基

2、酸袁含有一个磷脂酰乙醇胺结合蛋白(PEBP)结构域遥2基因和1基因的核苷酸相似性仅为56.7%袁二者蛋白的相似性为62.7%遥系统进化树分析表明2编码产物属于-like亚家族遥qRT-PCR分析表明袁2基因在不同的组织中均有表达袁在胚珠和茎中表达量较高遥半定量RT-PCR结果表明袁和1基因的表达模式不同袁2基因在刚萌发的种子中的表达量很低曰但随着ABA浓度的升高表达量显著提高袁并且ABA处理下萌发24h的种子中的表达量要高于72h中的表达量袁表明该基因的表达受ABA的调节袁可能在种子萌发过程中起重

3、要作用遥关键词院2基因曰PEBP曰成花素曰ABA中图分类号院S562.035文献标志码院A文章编号院1002-7807渊2014冤03-0197-071**1**1221*GuChao,GuoDanli,ZhangFeng,LiXueyuan,AiXiantao,HuangXianzhong(1.830011,)Thephosphatidylethanolamine-bindingprotein(PEBP)genefamilyispresentinangiospermswiththreesubfa

4、milies,-like,1-likeand-like,whichhavingbeenshowntoactaskeyregulatorstocontrolgrowthanddifferentiation.Com-paredwiththeothertwokinds,theexactmolecularfunctionof-likesubfamilygenesisstillnotclear.One(1)-likehomologwasisolatedfromL.cv.Xinhai14throughRT-P

5、CRandRACEmethod,whichwasdesignated2gene(GenBankaccessionnumber:KF739071).Theopenreadingframe(ORF)of2is519bpinlength,encodingaputativeproteinof172aminoacids袁containingaPEBPconservedmotif.Sequencealignmentshowedthatthenucleotideof2genehasonly56.7%simila

6、ritywith1geneandtheaminoacidsofGbMFT2proteinhas62.7%similaritywithGbMFT1protein.PhylogeneticanalysisrevealedthatGbMFT2belongtothe-likeclade.ExpressionanalysisbyqRT-PCRindicatedthat2displayedamuchbroaderexpressionrange,withamaximumexpressioninstemandov

7、ule.Expressionanalysisbysemi-quantitativeRT-PCRmethodindicatedthat2ex-pressionlevelsingerminatingseedswerelow,butgraduallyelevatedwhentreatedalongwithincreasedconcentrationsofABA.Ourresearchshowedthattheexpressionof2wasup-regulatedbyABAingerminatingse

8、eds,showingsignificantdiffer-encescomparedwith1inthecaseofABAtreatment.2gene;PEBP;florigen;ABA拟南芥中磷脂酰乙醇胺结合蛋白(Phos-族包含6个成员袁分别是院[1-3][2-4]phatidylethanolamine-bindingproteins袁PEBPs)家()尧1(1)尧收稿日期院2013-11-25作者简介院顾超(1987-)袁男袁硕士研究生袁guchaomail@163.co