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1、中国水稻科学(ChinJRiceSci),２０１５,２９(１):７３－８１http://www．ricesci．cnDOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１５．０１．００９７３水稻糖质胚乳突变体SugＧ１１籽粒灌浆过程的淀粉合成关键酶活性及其与淀粉理化特性关系赵华１,３王俊敏２张其芳３赵倩３梅淑芳１刘向蕾１程方民３,∗(１金华职业技术学院,浙江金华３２１０１７;２浙江省农业科学院作物与核技术研究所,杭州３１００３１;３浙江大学农业与生物技术学院,杭州３１００５８;∗通讯联系人,EＧmail:chengfm＠zju．edu．cn)ActivitiesofSeve
2、ralStarchSynthesisEnzymesinFillingGrainsforRiceSugaryEndospermMutant(SugＧ１１)andItsRelationtoStarchQualityZHAOHua１,３,WANGJunＧmin２,ZHANGQiＧfang３,ZHAOQian３,MEIShuＧfang１,LIUXiangＧlei１,CHENGFangＧmin３,∗(１JinhuaPolytechnic,Jinhua３２１０１７,China;２ZhejiangAcademyofAgriculturalSciences,Hangzhou３１００３１,China;
3、３CollegeofAgricultureandBiotechnology,ZhejiangUniversity,Hangzhou３１００５８,China;∗Correspondingauthor,EＧmail:chengfm＠zjue．duc．n)ZHAOHua,WANGJunmin,ZHANGQifang,etal．Activitiesofseveralstarchsynthesisenzymesinfillinggrainsforricesugaryendospermmutant(SugＧ１１)anditsrelationtostarchquality．ChinJRiceSci
4、,２０１５,２９(１):７３Ｇ８１．Abstract:Withthericesugaryendospermmutant(SugＧ１１)anditswildＧtype(Zhonghua１１)asmaterials,thetotalsolublesugar,sucrosecontents,starchaccumulationinfillinggrainsandthestarchphysicochemicalpropertieswerecomparedtofurtherrevealthetemporalpatternsofsugarcontent,severalkeyenzymesacti
5、vitiesandtheirisoformgenestranscriptionalexpressionlevelduringthegrainfillingsoastoanalyzethephysiologicalmechanismforSugＧ１１whosesugarscontentschangeand１０００Ｇgrainweightdeclinefromthepointofstarchbiosynthesispathwayinricegrains．Theresultshowedthatthedifferencesinsolublesugarandsucrosecontentsbet
6、weenSugＧ１１anditswildＧtypewerenotobviousattheinitialstageofgrainfilling,butwiththefillingprocessitgraduallybecameapparent．Secondly,theamylose(Am)contentandBV(Am)ofSugＧ１１weresignificantlydecreasedwhilethestarchsolubilityandBV(amylopectin,Ap)wereincreasedcomparedwithZhonghua１１,indicatingthatthephy
7、sicochemicalpropertiesofgrainstarchweresignificantlyaffectedinthesugaryendospermmutant．Finally,amongthekeyenzymesinstarchsyntheticmetabolism,debranchingenzyme(DBE),existedinSugＧ１１seeds,werequitedifferentfromitswildtypeinactivityan
