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1、中国水稻科学(ChinJRiceSci),２０１４,２８(５):４５８－４６４http://www．ricesci．cn４５８DOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１４．０５．００２水稻叶片形态相关基因RL３(t)的遗传分析和精细定位郭旻李荣德姚健朱娟范祥云王伟汤述翥顾铭洪严长杰∗(扬州大学农学院/江苏省作物遗传生理国家重点实验室培育点/教育部植物功能基因组学重点实验室,江苏扬州２２５００９;∗通讯联系人,EＧmail:cjyan＠yzu．edu．cn)FineMappingofGeneRL３(t),ResponsibleforLeafShap
2、eFormationinRiceGUOMin,LIRongＧde,YAOJian,ZHUJuan,FANXiangＧyun,WANGWei,TANGShuＧzhu,GUMingＧhong,YANChangＧjie∗(KeyLaboratoryofCropGeneticsandPhysiologyofJiangsuProvince,KeyLaboratoryofPlantFunctionalGenomicsoftheMinistryofEducation,CollegeofAgriculture,YangzhouUniversity,Yangzhou２２５００９,Chi
3、na;∗Correspondingauthor,EＧmail:cjyan＠yzue．duc．n)GUOMin,LIRongde,YAOJian,etal．FinemappingofgeneRL３(t),responsibleforleafshapeformationinrice．ChinJRiceSci,２０１４,２８(５):４５８Ｇ４６４．６０Abstract:Twomutantswithrolledleaves,derivedfromtheindicacultivar９３Ｇ１１viatheradiationofCoＧγrayinM２generation,tempo
4、rallydesignatedasrl３(t)Ｇ１andrl３(t)Ｇ２,wereservedasmaterialsforexploringthemechanismunderlyingtherollingleafcharacteristic．Morphologicalanalysisshowedthat,thesetwomutantshavetypicallyadaxiallyrolledleaves．Inaddition,whencomparedwithwildtype９３Ｇ１１,theplantheightsandpaniclelengthsofrl３(t)Ｇ１a
5、ndrl３(t)Ｇ２significantlydecreased,aswellastheseedsettingpercentage．Cytologicalanalysissuggeststhattherolledleafphenotypemaybecausedbythechangeofnumberandsizeofbulliformcells．Geneticanalysisindicatedthatrolledleafcharacteriscontrolledbyarecessivenucleargene．Crossingbetweenthetwomutants,th
6、eF１[rl３(t)Ｇ１/rl３(t)Ｇ２]plantsexhibitrolledleaf,suggestingtheyareallelic．TomaptheRL３(t)gene,anF２populationwasgeneratedbycrossingtherl３(t)Ｇ１mutantswithWuyunjing８asamappingpopulation．Byusingsimplesequencerepeat(SSR)markersandsomenewdesignedsequencetaggedsite(STS)markers,RL３(t)wasinitiallyma
7、ppedintheregionbetweentheSTSmarkerM３Ｇ４５andtheSSRmarkerRM６６７６withthegeneticdistancesof５．５cMand４．４cM,respectively,onthelongarmofchromosome３．Furthermore,withtheenlargedpopulationandmoredevelopedSTSmarkers,RL３(t)genewasfinallydelimitedtoa４６ＧkblongregiongovernedbytheSTSmarkersS３Ｇ３９a
