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1、棉花学报CottonScience2016,28(3):208―214植酸酶基因对陆地棉产量性状的影响*柯会锋袁王省芬袁吴立强袁李志坤袁张艳袁张桂寅袁马峙英渊河北农业大学/教育部华北作物种质资源研究与利用重点实验室袁河北保定071001冤摘要院在人工控制渊培养基尧水培尧沙培冤条件下袁植酸酶基因具有分解利用培养基质植酸磷尧提高磷素利用效率的功能袁但在田间的表现目前尚未明确遥在前期完成的转基因陆地棉新材料盆栽试验基础上袁将其种植在田间条件下袁研究基因对棉花产量性状的影响遥结果表明袁部分转基因棉花新材料的单株结铃数尧铃重尧衣分尧籽棉产量尧皮棉产量与野生型对照存在显著差异袁在田间条件下
2、具有改良转基因棉花部分产量性状的能力遥在此基础上袁遴选出2个转棉花优良新品系G3尧G2袁可用作今后转植酸酶基因棉花新品种培育的基础材料遥关键词院植酸酶基因曰田间条件曰陆地棉曰产量性状曰植酸磷中图分类号院S562.03文献标志码院A文章编号院1002-7807渊2016冤03-0208-0710.11963/issn.1002-7807.201603003*KeHuifeng,WangXingfen,WuLiqiang,LiZhikun,ZhangYan,ZhangGuiyin,MaZhiying(071001,)Phosphorus(P)isanimportantnutrien
3、tforplantgrowth,andonlytheinorganicform(Pi)dissolvedinsoilisreadi-lyavailableforuptake.However,abundantPexistsinthesoilasorganicP(Po),ofwhichmorethan50%isphytate.Phytaseisaspecializedphosphatasethatcatalyzesthesequentialhydrolysisofphytatetoproducelessphosphorylatedmyo-inositolderivativesand
4、inorganicphosphate.Inrecentyears,numerousphytasegeneshavebeendemonstratedtobeimportantforuti-lizingPifromphytateintransgenicplantsundercontrolledconditions,suchasinsolidnutritionmedium,hydroponicmedi-um,sterilizedvermiculite,andsandculture.However,thecapacityoftransgenicplantstoaccessphospha
5、tewhengrowninthefieldhasremainedunclear.Ourpreviousstudyobtainedsometransgeniccottonlinescontainingthephytasegene,andtheirabilitytoutilizeorganicphosphateunderhydroponicandvermiculiteculturewasdemonstrated.Usingthese-overexpressionlinesunderfieldconditions,10yield-relatedtraitswereinvestigat
6、edandanalyzedtodeterminethein-fluenceofthegeneonyieldcharacteristicsunderfieldconditions.Thisrevealedthenumbersofbollsperplant,bollweight,lintpercentage,seedcottonyield,andthelintyieldofpartiallytransgeniccottonlinesweresignificantlyhigherthanobservedinthewild-typecontrol.Basedontheseyieldtr
7、aits,twoelitetransgeniccottonlines,G3andG2,wereselectedfortheirhigheryieldcharacteristics.Theselineshavegreatpotentialforuseinfuturetransgeniccottonbreedingprograms.phytasegene;fieldconditions;uplandcotton;yieldcharacteristics;phytate土壤磷有50%～80%以非有
