普通小麦-卵穗山羊草种质的菲利普孢囊线虫抗性

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1、作物学报ACTAAGRONOMICASINICA2014,40(11):19561963http://zwxb.chinacrops.org/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2014.01956普通小麦–卵穗山羊草种质对菲利普孢囊线虫的抗性**邢小萍杨静袁虹霞张佳佳李洪连刘文轩河南农业大学/河南省粮食作物协同创新中心/小麦玉米作物学国家重点实验室,河南郑州450002摘要:菲利普孢囊线虫(Heteroderaf

2、ilipjevi)是我国黄淮麦区新发现的一种病原线虫,但在小麦属中缺乏有效抗源。为从小麦近缘种属中发掘抗病资源,采用室内接种平均单株雌虫鉴定法和相对抗病指数法,从34份卵穗山羊草(AegilopsgeniculataRoth)材料中筛选出6份抗H.filipjevi的种质材料,其中PI542187表现高抗,PI564186、PI573396、gPI374365、PI361880和PI374365表现抗病。对中国春–卵穗山羊草染色体附加系抗性鉴定,发现卵穗山羊草7U和gg5M附加系的单株白雌虫数明显低于中国春。连续两年又对17份小麦–卵

3、穗山羊草5M-5D易位系材料进行抗性鉴ggg定现发,5M464、5M466和5M457抗性较好。关键词:卵穗山羊草;菲利普孢囊线虫;附加系;易位系;抗性鉴定ResistancetoHeteroderafilipjeviinTriticumaestivum–AegilopsgeniculataGermplasm**XINGXiao-Ping,YANGJing,YUANHong-Xia,ZHANGJia-Jia,LIHong-Lian,andLIUWen-XuanHenanAgriculturalUniversity/Collaborat

4、iveInnovationCenterofHenanGrainCrops/NationalKeyLaboratoryofWheatandMaizeCropScience,Zhengzhou450002,ChinaAbstract:HeteroderafilipjeviisapathogenicnematodeofwheatnewlydiscoveredinHuang-HuaiPlainofChina.However,germplasmresistanttoH.filipjevihasbeenrarelyfoundingenusTrit

5、icum.Tomineresistancegenesfromwheatrelativespecies,weidentifiedtheresistancetoH.filipjeviin34accessionsofAegilopsgeniculatabyinoculatingH.filipjeviXuchangpopulationwiththemeanfemalenumberperplantmethodandrelativeresistanceindexmethodsingreenhouse.Sixcandidatesofgermplas

6、mwereidentifiedincludingPI542187inhighresistanceandotherfiveaccessions(PI564186,PI573396,PI374365,PI361880,andPI374365)inresistance.AsetofChineseSpring(CS)–Ae.geniculataadditionlineswereusedtochromosomallylocatetheresis-ggtancegene(s).Lines7Uand5Mshowedobviouslyfewerfem

7、alesperplantthanCS.Intwoconsecutiveyears,17CS–Ae.geni-ggculata5Mg-5DtranslocationlineswerefurthertestedforresistanceagainstH.filipjevi.Lines5M464,5M466,and5Mg457exhibitedresistancetoH.filipjevi.Keywords:Aegilopsgeniculata;Heteroderafilipjevi;Additionalline;Translocation

8、line;Resistanceidentification[4]禾谷孢囊线虫病是一种世界性土传线虫病害,首次发现,经鉴定,病原为H.avenae,随后在10[5-6]可危害小麦属(Triticum)、大麦属(Hordeu