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1、中国水稻科学(ChinJRiceSci),2017,31(4):364－370364http://www.ricesci.cnDOI:10.16819/j.1001-7216.2017.7017基于重测序的染色体片段代换系定位水稻抽穗期QTL1,221122222,*王军朱金燕陶亚军周勇范方军李文奇王芳权仲维功杨杰1,*梁国华12(扬州大学江苏省粮食作物现代产业技术协同创新中心/教育部植物功能基因组学重点实验室,江苏扬州225009；江苏省农业科学院粮食作物研*究所/国家水稻改良中心南京分中心,南京210014;通讯联系人,E-mail:ricegb@
2、yzu.edu.cn)MappingofQTLsforHeadingDateUsingWhole-genomeRe-sequencedChromosomeSegmentSubstitutionLinesinRice1,2211222WANGJun,ZHUJinyan,TAOYajun,ZHOUYong,FANFangjun,LIWenqi,WANGFangquan,22,*1,*ZHONGWeigong,YANGJie,LIANGGuohua1(JiangsuCo-InnovationCenterforModernProductionTechnolog
3、yofGrainCrops/KeyLaboratoryofPlantFunctionGenomics,MinistryofEducation2YangzhouUniversity,Yangzhou225009,China;InstituteofFoodCrops,JiangsuAcademyofAgriculturalSciences/NanjingBranchofChineseNational*CenterforRiceImprovement,Nanjing210014,China;Correspondingauthor,E-mail:ricegb@
4、yzu.edu.cn)Abstract：【Objective】Headingdateisanimportantagronomictraitinrice,itisatypicalquantitativetraitcontrolledbymultiplegenes.Asnovelresearchmaterial,chromosomesegmentsubstitutionlinesareusefulinQTLfinemappingandcloningbecauseofminimizingtheinterferenceofgeneticbackgroundam
5、ongplants.【Method】Inthisstudy,128whole-genomere-sequencedchromosomesegmentsubstitutionlinesderivedfromNipponbareasdonorparentinthebackgroundof9311,wereusedformappingQTLsforheadingdatebycombiningthesequencing-basedBin-mapwithmultiplelinearregressionanalysis.【Result】SixQTLsforhead
6、ingdatewereidentifiedintwoenvironmentsandtwoyears.qHD2.1wasmappedintheregionof759848bponthechromosome2;qHD2.2wasmappedintheregionof45286bponthechromosome2;qHD3.1wasmappedintheregionof147931bponthechromosome3;qHD5.1wasmappedintheregionof213351bponthechromosome5;qHD5.2wasmappedint
7、heregionof442305bponthechromosome5;qHD8.1wasmappedintheregionof538176bponthechromosome8.【Conclusion】TheresultsareimportantfortheQTLscloningandprovideafoundationforunderstandingmolecularregulationmechanismofheadingdateinrice.Keywords:rice;chromosomesegmentsubstitutionlines;re-seq
8、uenced;headingdate摘要：【目的】水稻的抽穗期是决定水稻产量及其适用性的重要农
