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1、棉花学报CottonScience2014袁26渊6冤院499~505利用染色体片段代换系定位棉花抗黄萎病QTL1111*2*赵君袁刘剑光袁吴巧娟袁肖松华袁袁有禄渊1.江苏省农业科学院经济作物研究所/农业部长江下游棉花和油菜重点实验室袁江苏南京210014曰2.中国农业科学院棉花研究所/棉花生物学国家重点实验室袁河南安阳455000冤摘要院通过陆地棉与海岛棉杂交并与陆地棉回交袁获得1个染色体片段代换系苏VR043袁抗江苏非落叶型黄萎病菌系BP2遥分子检测表明袁苏VR043兼有陆地棉苏棉8号的遗传背景和海7124的D4染色体片段遥
2、以苏VR043和苏棉8号为亲本构建包含176个单株的F2群体袁通过标记分析和F2:3家系抗病鉴定袁发现抗病性状与标记NAU3392连锁袁值为2.3伊10-12遥根据棉花D组染色体测序结果袁参照置换区段的基因组序列袁合成92对SSR引物曰PCR扩增分析发现袁有5对引物在苏棉8号和苏VR043之间表现多态性袁并将抗病主效QTL定位在标记ZHX32和NAU3392之间袁标记间遗传距离为3.2cM袁QTL解释表型变异64.8%遥同时参照棉花D组测序结果袁提取对应的置换区段序列袁预测包含63个基因袁基因聚类分析表明7个基因参与抗逆反应袁其
3、中1个基因与抗病相关遥关键词院棉花曰黄萎病曰分子标记曰抗病基因曰QTL中图分类号院S562.03文献标志码院A文章编号院1002-7807(2014)06-0499-071111*2*ZhaoJun,LiuJianguang,WuQiaojuan,XiaoSonghua,YuanYoulu(1.210014,;2.455000,)TodissecttheVerticilliumwiltresistanceQTLandfindmolecularmarkerscloselylinkedwiththeresistancetrait,w
4、ecreatedanewmaterial.BycrossingL.cv.Sumian8astherecipientparentandL.cv.Hai7124asthedonorparent,wegeneratedachromosomesegmentintrogressionline(CSIL),SuVR043,withhigherresis-tancetonon-defoliatingBP2isolatesof.SuVRo43wascharacterizedascarryingonehomozygouschromo-some22
5、(D4)segmentrepresentedbytheSSRmarkerNAU3392fromcv.Hai7124inthebackgroundoftheup-landcottonSumian8.WeconstructedanF2mappingpopulation,SuVR043伊Sumian8,containing176individuals.BasedonSSRmarkersandFphenotypesforresistancetowiltstrainBP2,singlemarkeranalysisshowedtheresi
6、stancephe-学报页脚2:3notypewaslinkedtothemakerNAU3392withavalueof2.3伊10-12.UsingtherecentlyreleasedcottonDgenomesequencein-formationasareference,weextractedtheintrogressionsegmentsequenceanddesigned92SSRprimerpairs,fromwhichfivepolymorphicSSRlociweredetectedbetweenSuVR04
7、3andSumian8andusedtomaptheresistanceQTLontheintrogressionsegment.TheQTLwasanchoredbetweentheZHX32andNAU3392lociwithadistanceof3.2cM,andex-plained64.8%ofthephenotypicvariation.Moreover,wepredicted63candidategenesusingthecottonDgenomesequencein-formation.TheresultsofGe
8、neOntologyanalysisindicatedthatthesegeneswereinvolvedindifferentbiologicalprocesses,withsevenofthemparticipatinginthestresssignaltr
