资源描述:
《MicroRNA和lncRNA在晶状体发育和白内障形成中的作用.pdf》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、中国科技论文在线http://www.paper.edu.cnMicroRNA和lncRNA在晶状体发育和白内障形成中的作用**郑嘉琳,孙靖,蔡思维,张红,张琰5(天津医科大学眼科医院,天津医科大学眼科研究所,天津医科大学眼视光学院,天津300384)摘要:微小RNA(microRNA,miRNA)和长链非编码RNA(longnon-codingRNA,lncRNA)均属于非编码RNA。MiRNA是一段由19-22个核苷酸组成的高度保守单链非编码RNA,10它可以特异性结合目标mRNA的3’非翻译区,从而诱导目标mRNA降解或者抑制其翻译,最终影响细胞增殖、分化以及
2、凋亡等生物功能;lncRNA是一段长度为200bp~100kb的单链非编码RNA,它能调节细胞分裂、生长、分化以及凋亡等重要的生物学过程。研究表明miRNA或lncRNA的异常表达可导致晶状体正常发育过程紊乱、晶状体上皮细胞凋亡,纤维细胞排列混乱,晶状体透明度降低,从而导致白内障的发生。本文主要总结七种15miRNAs(miR184、miR204、let7、miR29、miR16,miR125,miR34a)以及两种lncRNAs(lncRNA-MIAT、LOXL1-AS1)在晶状体发育和白内障形成中的作用机制,为寻找白内障防治的新靶点提供思路。关键词:微小RNA;
3、长链非编码RNA;白内障;晶状体发育;上皮间质转化中图分类号:R7720RoleofmicroRNAandlncRNAinlensdevelopmentandcataractformationZHENGJialin,SUNJing,CAISiwei,ZHANGHong,ZHANGYan(TianjinMedicalUniversityEyeHospital,TianjinMedicalUniversityEyeInstitute,Collegeof25OptometryandOphthalmology,TianjinMedicalUniversity,Tianjin
4、300384,China)Abstract:MicroRNA(miRNA)andlongnoncodingRNA(lncRNA)belongtononcodingRNA.MiRNAisa19-22ntlong,highlyconserved,single-strandednoncodingRNA.ThemiRNAcanspecificallybindtothe3’untranslatedregionofthetargetmRNA,inducethetranscriptdegradationortranslationinhibition,andeventuallyim
5、pactthebiologicalfunctionsofcell,includingproliferation,30differentiation,andapoptosis.WhereaslncRNAisa200bp-10kblong,single-stranded,noncodingRNA,whichcanregulatetheimportantbiologicalprocesses,includingcelldivision,growth,differentiation,andapoptosis.Researchhasdemonstratedthattheabe
6、rrantexpressionofmiRNAorlncRNAresultsindisruptionofnormallensdevelopment,apoptosisoflensepithelialcells,disarrangementoflensfibrocells,andthereducedlenstransparency,therebycausingtheincidenceof35cataract.Thisreviewsummarizestheeffectsandthemechanismsof7miRNAs(miR184,miR204,let7,miR29,m
7、iR16,miR125,miR34a)and2lncRNAs(lncRNA-MIAT,LOXL1-AS1)duringlensdevelopmentandcataractformation,inthehopethatitcouldprovideinsightsforthenovelinterventionalandtherapeutictargetsforcataract.Keywords:miRNA;lncRNA;cataract;lensdevelopment;EMT400引言晶状体是位于眼前节的弹性、透明的双凸结构,由晶状体上皮细胞和晶状体纤维细胞组成。晶