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1、中国科技论文在线http://www.paper.edu.cnGenome-wideidentificationandexpressionanalysisofthepolyaminebiosynthesisgeneinsweetorange(Citrus5sinensis)WuHao,LiuJihong**(CollegeofHorticultureandForestrySciences,HuazhongAgriculturalUniversity,)Abstract:Polyamines(PAs)arelowmolecula
2、rweight,aliphaticpolycationsfoundinthecellsofalllivingorganisms.Andinplants,agrowingnumberofevidencessupportthatPAsplayimportantroles10inabioticstresses.Inthisstudy,atotalof18polyaminebiosynthesisgeneswhichbelongto10kindsofdifferentpolyaminebiosynthesisenzymeswereis
3、olatedfromtheentirecitrusgenomeandafurtheranalysisincludingthechromosomallocations,phylogeneticrelationships,functionalannotations,promoteranalysis,andgenestructureswereperformed.Tissuespecificexpressionofthesegeneswasdetectedinroot,stem,leaf,pulp,peel,andcallus.The
4、polyaminebiosynthesisgenedisplayedvarious15responsestoexogenouspolyamines(putrescine,spermidine,spermine)andABAtreatments,andweredifferentiallyalteredbyabioticstresses,includingcoldandsalt.Andthechangepatternsofthreemainpolyaminesduringcoldstressinleavesandcalluswer
5、echaracterized.Thecomprehensiveanalysisofpolyaminebiosynthesisgeneishelpfultoexploitstrategiestoimproveplanttolerancetomultipleenvironmentalstresses.20Keywords:Sweetorange(Citrussinensis);polyaminebiosynthesisgene;abioticstress;Geneexpression0IntroductionPolyamineis
6、anorganiccompoundhavingtwoormoreprimaryaminogroups–NH2.Thereare25threemajorpolyaminesinplants,putrescine(Put),spermidine(Spd),andspermine(Spm)[1],whichplayimportantrolesinvariousprogressesofplantgrowthanddevelopment,includingcellproliferation[2],morphogenesis[3],dif
7、ferentiation[4],programmedcelldeath[5],andresponsestovariousenvironmentalstresses[6-8].Andtheplantstresstolerancewouldbeimprovedwhentreatedwithexogenouspolyamines.Forexample,sprayingputrescinecouldreduce30flooding-inducedoxidativedamagebyincreasingtheantioxidantprop
8、erties[9].Exogenousspermidinemaydifferentiallyaltertheactivitiesofsomescavengingsystemenzymes,hydrogenperoxideandsuperoxideradicallevelsin
