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大小:443.86 KB
页数:7页
时间:2022-12-24
《KJ 202106 玉米及其碾磨加工品中伏马毒素的快速检测 胶体金免疫层析法》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
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1犓犑202106!"#$%&'()*+,-./0123456789:;1<=!"#$%&'()*+,-./01234B1、1234B2、1234B3(56789FB1、FB2、FB3):;<=>?@ABCD#。!"#EFG'()*+,-./01234(5FB1、FB2、FB3HIJ)K:;%L=%。2>?!"#MNOPQRABCDST。U/0K1234(FB1、FB2、FB3)VWXYZ[\],\][VU/^_[^_`,a>?@bcKdeLf?gh,QRf?ijkl0<=m(Tm)nfSgh,opqrjkl<=m(Tm)stuv。wx<=m(Tm)ayRm(Cm)stz{|},~U/01234(5FB1、FB2、FB3HIJ)%L%。3@ABCD$%,!"#Fj9D,Y9GB/T6682$%KY。3.1@A3.1.1WX(CH3CN)。3.1.2(C4H11NO3),Tris。3.1.3(HCl,37%)。3.1.420(C58H114O26)。3.2EFGH1234K0、、CAS¡、¢£、¤~¢I¥¦1,§≥95%。I:¨©ª«¬。J1+,-.EFGH/*KLM、NKLM、犆犃犛O、PQR、STPQHU0CAS¡¢£¤~¢I1234B1FumonisinB1(FB1)116355830C34H59NO15721.831234B2FumonisinB2(FB2)116355841C34H59NO14705.831234B3FumonisinB3(FB3)136379594C34H59NO14705.833.3VWXY3.3.1WXYZ[(50+50):®I]100mLWX(3.1.1)i100mLY,¯h°。1
2犓犑2021063.3.2^Z[(2mol/L):I]12mL±(3.1.3)-²³60mLY,¯h°。3.3.3U/^_[:]12.1gTris(3.1.2)G800mLY0,´µZ¶`,-²EI^Z[(3.3.2)·¸pH¹8.5,º-²5g20(3.1.4)»´µ¯°,FY%¼¹1000mL。3.4Z[VWXY3.4.11234B1b½¾¿Z[(100μg/mL):½À]FB110mg(ÁÀ¹0.01mg)¹ÂÃ0,FWXYZ[(3.3.1)Z¶,»ÄŹ100mL¼IÆ0,%¼¹Ç§。ÈZ[ÉÊ`ËÌ-20℃ÍÎ。ÏÐ6ÑÒ。3.4.21234B2b½¾¿Z[(100μg/mL):½À]FB210mg(ÁÀ¹0.01mg)¹ÂÃ0,FWXYZ[(3.3.1)Z¶,»ÄŹ100mL¼IÆ0,%¼¹Ç§。ÈZ[ÉÊ`ËÌ-20℃ÍÎ。ÏÐ6ÑÒ。3.4.31234B3b½¾¿Z[(100μg/mL):½À]FB310mg(ÁÀ¹0.01mg)¹ÂÃ0,FWXYZ[(3.3.1)Z¶,»ÄŹ100mL¼IÆ0,%¼¹Ç§。ÈZ[ÉÊ`ËÌ-20℃ÍÎ。ÏÐ6ÑÒ。3.4.4¯hb½.ÓZ[:½ÀI]FB1b½¾¿Z[(3.4.1)14mL、FB2b½¾¿Z[(3.4.2)4mL、FB3b½¾¿Z[(3.4.3)2mL¹ªÔ50mL¼IÆ0,-WXYZ[(3.3.1)%¼¹Ç§,Õ³FB1I±§928μg/mL、FB2I±§98μg/mL、FB3I±§94μg/mLK¯hb½.ÓZ[。ÈZ[ÉÊ`ËÌ4℃ÍÎ。ÏÐ6ÑÒ。3.4.5Öb½Z[9×Ø]Ù,ÚT)ÛFÜÝh¤Þ$%。3.5CD1234>?@ABCDjß,Ôàáâ@bãä、>?@jkl,EFG'()*+,-./。4\]^_`4.1å¢æç:èI®90.01gi0.01mg。I:éêëì«ØÕÝh$%Kb½Z[Ù,íîï¿èI90.01mgKæç。4.2ðñò。4.3óôõ:Ä;≥4000r/min。4.4Å[ò:Iö®9100μL、200μL、1mL、5mL。4.5÷øò。4.6ùú¯hò。4.7pHJ。4.8ûüò:«y20℃~25℃。4.9>?@ýþÿ(«!)。5abcd§15℃~35℃,¤~"§≤80%。6P:ef6.1@gY`#U/$%#&¹'1kg,Fðñò,ñ¹(×wx)ä*+,G1mmK-¢,¯°`.2
3犓犑202106/0,®1²23¼òÓ9jUi4U。jU5=,4U67G200g5¿8<。jUi4UÉÊ`b9,»:G4℃6ËÌÍÎ。6.2@ghi6.2.1!"%&'()½À]jU5g(ÁÀ¹0.01g)G50mLóôÚ0,-²20mLWXYZ[(3.3.1),÷ø\]5min¨ùú\]2min,G4000r/minóô3min¨;:5minÛ<[C,=>]100μLn?[-²³1600μLU/^_[(3.3.3)KóôÚ0,¯°`>95=[。6.2.2!"½À]jU5g(ÁÀ¹0.01g)G50mLóôÚ0,-²20mLWXYZ[(3.3.1),÷ø\]5min¨ùú\]2min,G4000r/minóô3min¨;:5minÛ<[C,=>]Ô%In?[aWXYZ[(3.3.1)51∶1?@¯h°,º]100μL¯hZ[-²³1600μLU/^_[(3.3.3)KóôÚ0,¯°`>95=[。6.33jefFÅ[òA]200μL5=[G@bãä0,B8CA4~5D,Ûãäj¯h°,Gûüò020℃~25℃ûü3min,#jkl6EF²³@bãäZ[G×,Gûüò020℃~25℃BÜ5min,HIjkl,JK6EU/L,M1min~3minNýgO。6.4Hk@l6.4.1PQU/ܪÙRSjëi-byjë。Ø]ªTKRSjU,V|"#<=1234(5FB1、FB2、FB3HIJ)âI,G200μg/kg。6.4.2RSjë:]RSjU,$U6.2i6.3VWaU/ª#XÓ。6.4.3-byjë:½À]RSjU5g(ÁÀ¹0.01g)G50mLóôÚ0,'(U/0-²500μL¯hb½.ÓZ[(3.4.4),ÛFB1、FB2、FB3KâI®92.8mg/kg、0.8mg/kg、0.4mg/kg;'(+,-./U/0-²250μL¯hb½.ÓZ[(3.4.4),ÛFB1、FB2、FB3KâI®91.4mg/kg、0.4mg/kg、0.2mg/kg。$U6.2i6.3VWaU/ª#XÓ。7mnojpqMN1234>?@ABCDjß!Fý"#。YFZ[#~gOý,Z[%\]^_^1i^2\。I:`«ÛF>?@ýþÿý,ýþÿKa?XÓaýSbcUýþÿKÛFdef。7.1rs;7.1.1tuyRm(Cm)6gt,¦ehiXÓ¨jklíÏ。7.1.2vwmn<=m(Tm)6gt¨<=m(Tm)st|yRm(Cm)st{,¦eU/01234HâIj3
4犓犑202106G"#<=k,9lL。7.1.3xwmn<=m(Tm)st|yRm(Cm)stz¨m<=m(Tm)stayRm(Cm)st¤é,¦eU/01234HâInG"#<=k,9oL。y1z{oj|}y(rs;)7.2~;7.2.1tuyRm(Cm)6gt,¦ehiXÓ¨jklíÏ。7.2.2vwmnyRm(Cm)gt,<=m(Tm)6gt,¦eU/01234HâIjG"#<=k,9lL。7.2.3xwmn<=m(Tm)ayRm(Cm)gt,¦eU/01234HâInG"#<=k,9oL。y2z{oj|}y(~;)4
5犓犑2021067.3Hk@lpqRSjë=%gOÜ9oL,-byjë=%gOÜ9lL。8mé<=gO9lLÙ,YF|"#Àp。9wZ9.1LqrbJs"#$UtuAv。9.26犓犑202106犃(
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