资源描述：
《荞麦花叶总黄酮对大鼠2型糖尿病胰岛素抵抗的影响》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、荞麦花叶总黄酮对大鼠2型糖尿病胰岛素抵抗的影响作者：尚保华，韩淑英，郑丽坤，朱丽莎，杨远明，米文生，魏洪磊【摘要】 目的研究荞麦花叶总黄酮（TFBFL）对2型糖尿病胰岛素抵抗大鼠的血糖、血脂和对胰岛素抵抗（IR）的影响,并探讨其作用机制。方法以脂肪乳灌胃联合低剂量四氧嘧啶注射方法制作2型糖尿病大鼠胰岛素抵抗模型。将SD大鼠随机分为正常组，模型组，TFBFL高、中、低治疗组，罗格列酮（RSG）治疗组。连续灌胃6周后,测定空腹血糖(FBG),糖耐量（OTGG），空腹胰岛素（FINS），血清总胆固醇（TC），甘油三酯（TG），游离脂肪酸(FFA),糖化血红蛋白（GHb），计算胰岛素敏
2、感指数(ISI)和KITT。HE染色观察胰腺和肾组织形态学改变。结果与正常对照组比较,模型组大鼠FBG，OTGG，FINS,TG,TC,FFA,GHb明显升高（P【关键词】 荞麦花叶黄总酮2型糖尿病胰岛素抵抗 Abstract:ObjectiveTostudytheeffectsoftotalflavonesofbuckwheatflowersandleavesonserumglucose,serumlipidandinsulinresistanceintype2diabeticratswithinsulinresistanceandtrytofinditsmechanism.M
3、ethodsThemodelofdiabetictype2ratswithinsulinresistancewasmadebyinjectingalloxanandgivingagastricperfusionofintralipid. Ratswererandomlydividedintonormalgroup,modelgroup,TFBFL100,200and400mg·kg-1groupsandrosiglitazonegroup.Fastingbloodglucose(FBG),OGTT,fastinginsulin(FINS),insulinsensitivityine
4、x(ISI),KITT,freefattyacids(FFA),triglyceride(TG),totalcholesterol(TC),glycosylatedhemoglobin(GHb)weremeasuredafter6weeks.Thepancreas,fat,renalissueswerestainedbyHEsoastoobserveitsmorphologicchanges.ResultsFBG,FINS,TG,TC,FFA,GHbsignificantlyincreasedinthemodelgroupascomparedwiththoseinnormalgro
5、up,whileISIwassignificantlyreduced.FBG,FINS,TG,TC,FFA,GHbsignificantlyreducedintheTFBFLgroupascomparedwiththoseinmodelgroup,whileISIwassignificantlyincreasedindose-dependentmanner.TheeffectsofhighdoseTFBFLtreatedgroupweresimilartothoseofRSGgroup.HE-stainedorganizationswereobservedthatthemodelg
6、rouporganizationalstructureofthepancreasandkidneywereabnormal.ThosepathologicchangesindifferentdoseTFBFLgroupsweresignificantlyimproved.ConclusionTFBFLcanincreaseinsulinsensitivity,improveinsulinresistanceandprotectorgansoftype2diabeticrats. Keywords:Totalflavonesofbuckwheatflowera
