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1、AmericanJournalofBotany97(7):12291240.2010.NATURALHYBRIDIZATIONINTROPICALSPIKERUSHESOFELEOCHARISSUBGENUSLIMNOCHLOA(CYPERACEAE):E1VIDENCEFROMMORPHOLOGYANDDNAMARKERSJanKošnar2,6,JirˇíKošnar2,MiroslavaHerbstová2,3,PetrMacek2,4,EliškaRejmánková5,andMilanŠtech2
2、2DepartmentofBotany,FacultyofScience,UniversityofSouthBohemia,Braniovská31,37005ČeskéBudeˇjovice,CzechRepublic;3InstituteofPlantMolecularBiology,BiologyCentreoftheAcademyofSciencesoftheCzechRepublic,Braniovská31,37005ČeskéBudeˇjovice,CzechRepublic;4Institu
3、teofBotany,AcademyofSciencesoftheCzechRepublic,Dukelská135,37982Trˇebonˇ,CzechRepublic;and5DepartmentofEnvironmentalScienceandPolicy,UniversityofCaliforniaDavis,OneShieldsAvenue,Davis,California95616USAPremiseofthestudy:Naturalhybridizationrepresentsanimpo
4、rtantforcedrivingplantevolutionandaffectingcommunitystructureandfunctioning.Hybridizationmaybeoverlooked,however,amongmorphologicallyhighlyuniformcongeners.AnexcellentexampleofsuchagroupisEleocharissubgenusLimnochloa,whichhasnoreliablyprovenhybrids.Doesthi
5、sreflectbiologicalbarrierstointerspecificcrossesordifficultiesindetectingthehybrids?WetestedthehypothesisthathybridizationoccursamongsympatricEleochariscellulosa,E.interstincta,andE.mutatainnorthernBelize,CentralAmerica.Methods:Morphometricstudy(407plants)was
6、followedbyexaminationofinter-simplesequencerepeat(ISSR)polymor-phisms(44plants)andITSsequencevariation(33plants).Keyresults:TwoputativelyhybridmorphotypeswerediscernedE.cellulosa-resemblingandE.interstincta-resembling.DNAmarkersofE.cellulosaandE.interstinc
7、tadisplayedadditiveconstitutioninplantsfromoneE.cellulosa-resemblingpopulationonly.TheotherputativelyhybridpopulationscontainedISSRandITSmarkersofthespeciestheyresembledmor-phologically,severaluniqueISSRmarkers,andITSsequencesofanundescribedSouthAmericanLi
8、mnochloaentity.DNAmarkersofE.mutatawereabsentintheputativehybrids.Conclusions:Simultaneoususeofvarioustypesofmolecularmarkerscanovercomemanypitfallsofinvestigationsconcerninghybridizationamongcloselyrelatedan
