issr analysis reveals high intraspecific variation in rauvolfia serpentina l.

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1、BiochemicalSystematicsandEcology40(2012)192–197ContentslistsavailableatSciVerseScienceDirectBiochemicalSystematicsandEcologyjournalhomepage:www.elsevier.com/locate/biochemsysecoISSRanalysisrevealshighintraspecificvariationinRauvolfiaserpentinaL.–Ahighvaluemedicinalplanta,*aaPadmeshPandaramPilla

2、i,JayakumariSanakanSajan,KuttapettyManikantanMenon,abKuttanappillySudhakaraPanickerJayakumar,ApianSubramoniamaBiotechnology&BioinformaticsDivision,TropicalBotanicGardenandResearchInstitute,Palode,Thiruvananthapuram695562,Kerala,IndiabPhytochemistryandPhytopharmacologyDivision,TropicalBotanicG

3、ardenandResearchInstitute,Palode,Thiruvananthapuram695562,Kerala,IndiaarticleinfoabstractArticlehistory:RauvolfiaserpentinaL.isahighvaluemedicinalplanthavingwidedistributionintheReceived30March2011tropicsaswellasotherpartsoftheworld.ThirteenaccessionsofRauvolfiacollectedfromAccepted22October201

4、1differentlocationsintheWesternGhatregionofthepeninsularIndiawereanalyzedforAvailableonline18November2011geneticvariationusing15ISSRprimers.The91polymorphicampliconsgeneratedwerestatisticallyanalyzedusingWinDistandPopGenesoftwaretocalculatevariousdiversityKeywords:indices.Contrarytothegeneral

5、conceptoflowgeneticvariationassociatedwithMolecularmarkersinbreeders,R.serpentinaexhibitshighgeneticdiversityasthesimilarityindexvaluebasedBiodiversityonNeiandLi’ssimilaritycoefficientrangesfrom0.44to0.92withmeanvalueof0.70.ThisWesternGhatsissupportedbyotherdiversityparameterslikeDegreeofgenet

6、icdifferentiation(Gst),Geneflow(Nm),Nei’sgenediversity(h)andShannonindexofgeneticdiversity(I).Clusteranalysisshowedcharacteristicgroupingwhichisnotinaccordancewiththeirgeograph-icalaffiliation.TheresultindicatesthatISSRmarkersaresufficientlyinformativeinassessinggeneticdiversityacrosswildaccessi

7、onsofR.serpentina.Thehighgeneticdiversityobservedinthisotherwiseself-pollinatedplantentailstheneedforevolvingeffectiveex-situstrategyforlong-termconservationofthismedicinalherb.Ó2011ElsevierLtd.Allrightsreserved.1.IntroductionRauvolfiaserpenti